Tested Applications
| Positive WB detected in | HepG2 cells, human adrenal gland tissue, C6 cells, mouse liver tissue, mouse spleen tissue, rat liver tissue |
| Positive IP detected in | mouse spleen tissue |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14905-1-AP targets PSMC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6700 Product name: Recombinant human PSMC2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 84-433 aa of BC002589 Sequence: KQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN Predict reactive species |
| Full Name | proteasome (prosome, macropain) 26S subunit, ATPase, 2 |
| Calculated Molecular Weight | 49 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC002589 |
| Gene Symbol | PSMC2 |
| Gene ID (NCBI) | 5701 |
| RRID | AB_2172484 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35998 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PSMC2 is also named as MSS1 and belongs to the AAA ATPase family. PSMC2 is 1 of 6 putative ATPases contained within the regulatory complex. PSMC2 is first identified as a possible cellular factor that cooperates with the human immunodeficiency virus-1 (HIV-1) protein Tat, a potent activator of virus gene expression. The PSMC2 gene product works in association with the small subunit of mitoribosomes and seems to play some part in mitochondrial translation(PMID:8392589).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMC2 antibody 14905-1-AP | Download protocol |
| IHC protocol for PSMC2 antibody 14905-1-AP | Download protocol |
| IP protocol for PSMC2 antibody 14905-1-AP | Download protocol |
| WB protocol for PSMC2 antibody 14905-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PSMC2 antibody (Cat# 14905-1-AP) has accumulated 15 citations across journals including Gastroenterology, EMBO Journal, Pharmacological Research, Acta Pharmacologica Sinica, Frontiers in Immunology, International Immunopharmacology, Osteoporosis International, Cell Cycle, Calcified Tissue International, Cardiovascular Drugs and Therapy, Discovery Oncology, Pharmgenomics & Personalized Medicine, Oncology Letters, Cancer Biotherapy & Radiopharmaceuticals, and eLife. Research fields span oncology (GIST, nasopharyngeal carcinoma, pancreatic cancer, osteosarcoma), cardiovascular disease, bone metabolism, proteasome biology, inflammation, and reproductive biology.
| Species | Application | Title |
|---|---|---|
Gastroenterology Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors | ||
Pharmacol Res Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14 | ||
Acta Pharmacol Sin Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition | ||
EMBO J 20S proteasome-regulated proteostasis in ELVAs is critical for oocyte-to-embryo transition and female fertility. | ||
Front Immunol PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB. | ||
Int Immunopharmacol Tianhe Zhuifeng Gao reverses inflammatory response and attenuates bone/cartilage destruction in rheumatoid arthritis via PSMC2-RUNX2-COL1A1 axis based on transcriptional regulatory network analysis and experimental validation |
