PSMC2 Polyclonal antibody

• 

  WB analysis using 14905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human adrenal gland using 14905-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 14905-1-AP

  IP result of anti-PSMC2 (IP:14905-1-AP, 4ug; Detection:14905-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

• 

  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 14905-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMC2 antibody (14905-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• Featured Product
• KD/KO Validated

PSMC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14905-1-AP

Publications(13)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

26S proteasome AAA-ATPase subunit RPT1, 26S proteasome regulatory subunit 7, MSS1

---

Tested Applications

• Positive WB detected in: HepG2 cells, human adrenal gland tissue, C6 cells, mouse liver tissue, mouse spleen tissue
• Positive IP detected in: mouse spleen tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 5 publications below
• CoIP: See 1 publications below

Product Information

14905-1-AP targets PSMC2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6700

  Product name: Recombinant human PSMC2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 84-433 aa of BC002589

  Sequence: KQTLQSEQPLQVARCTKIINADSEDPKYIINVKQFAKFVVDLSDQVAPTDIEEGMRVGVDRNKYQIHIPLPPKIDPTVTMMQVEEKPDVTYSDVGGCKEQIEKLREVVETPLLHPERFVNLGIEPPKGVLLFGPPGTGKTLCARAVANRTDACFIRVIGSELVQKYVGEGARMVRELFEMARTKKACLIFFDEIDAIGGARFDDGAGGDNEVQRTMLELINQLDGFDPRGNIKVLMATNRPDTLDPALMRPGRLDRKIEFSLPDLEGRTHIFKIHARSMSVERDIRFELLARLCPNSTGAEIRSVCTEAGMFAIRARRKIATEKDFLEAVNKVIKSYAKFSATPRYMTYN

• Full Name: proteasome (prosome, macropain) 26S subunit, ATPase, 2
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC002589
• Gene Symbol: PSMC2
• Gene ID (NCBI): 5701
• RRID: AB_2172484
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35998
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC2 is also named as MSS1 and belongs to the AAA ATPase family. PSMC2 is 1 of 6 putative ATPases contained within the regulatory complex. PSMC2 is first identified as a possible cellular factor that cooperates with the human immunodeficiency virus-1 (HIV-1) protein Tat, a potent activator of virus gene expression. The PSMC2 gene product works in association with the small subunit of mitoribosomes and seems to play some part in mitochondrial translation(PMID:8392589).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human		Pharmacol Res; Curcumin modulates β-catenin stabilization via targeting proteasomal deubiquitinating enzyme USP14; Authors - Mengzhen Shen
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
rat	WB	Cardiovasc Drugs Ther; Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.; Authors - Kuixiang Chen
mouse	WB,IHC	Calcif Tissue Int; Silencing Proteasome 26S Subunit ATPase 2 (PSMC2) Protects the Osteogenic Differentiation In Vitro and Osteogenesis In Vivo.; Authors - Bin Zhou ; KD Validated
human	IHC	Oncol Lett; PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.; Authors - Yushen Su