PSMC3 Polyclonal antibody

PSMC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 24142-1-AP

Synonyms

TBP-1, TBP1, Tat-binding protein 1, 26S proteasome regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5

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Validation Data Gallery

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  WB analysis using 24142-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 24142-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24142-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24142-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24142-1-AP

  IP result of anti-PSMC3 (IP:24142-1-AP, 4ug; Detection:24142-1-AP 1:800) with HeLa cells lysate 3200ug.

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  IHC staining of human ovary tumor using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 24142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PSMC3 antibody (24142-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A431 cells, HeLa cells, HepG2 cells, Jurkat cells, MCF-7 cells, T-47D cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human ovary tumor tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 4 publications below

Product Information

24142-1-AP targets PSMC3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMC3 fusion protein Ag21408
• Full Name: proteasome (prosome, macropain) 26S subunit, ATPase, 3
• Calculated Molecular Weight: 49 kDa
• Observed Molecular Weight: 49 kDa
• GenBank Accession Number: BC008713
• Gene Symbol: PSMC3
• Gene ID (NCBI): 5702
• RRID: AB_2879440
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMC3(26S protease regulatory subunit 6A) is also named as TBP1, Proteasome 26S subunit ATPase 3 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. It is a multifunctional protein, component of the regulatory subunit of the proteasome, involved in different cellular processes.

Publications

Species	Application	Title
human	WB	ChemMedChem; Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity.; Authors - Anna Maria Santoro
human,mouse	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan
human	WB	Mol Biol Cell; Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants; Authors - Eli Fritz McDonald
human	WB	Hum Mol Genet; PSMC5 insufficiency and P320R mutation impair proteasome function; Authors - Zhong-Qiu Yu