CoraLite® Plus 488-conjugated PSMD11 Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated PSMD11 Recombinant monoclonal antibody (CL488-86596-3)

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  IF Staining of MCF-7 using CL488-86596-3

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 PSMD11 antibody (CL488-86596-3, Clone: 251499B5 ) at dilution of 1:200, CL594-phalloidin (red).

PSMD11 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-86596-3

Clone No.251499B5

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

26S proteasome non-ATPase regulatory subunit 11, p44.5, Rpn6, S9

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Tested Applications

• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-86596-3 targets PSMD11 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6435

  Product name: Recombinant human PSMD11 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 76-422 aa of BC000437

  Sequence: FLNSISKAKAARLVRSLLDLFLDMEAATGQEVELCLECIEWAKSEKRTFLRQALEARLVSLYFDTKRYQEALHLGSQLLRELKKMDDKALLVEVQLLESKTYHALSNLPKARAALTSARTTANAIYCPPKLQATLDMQSGIIHAAEEKDWKTAYSYFYEAFEGYDSIDSPKAITSLKYMLLCKIMLNTPEDVQALVSGKLALRYAGRQTEALKCVAQASKNRSLADFEKALTDYRAELRDDPIISTHLAKLYDNLLEQNLIRVIEPFSRVQIEHISSLIKLSKADVERKLSQMILDKKFHGILDQGEGVLIIFDEPPVDKTYEAALETIQNMSKVVDSLYNKAKKLT

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 11
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC000437
• Gene Symbol: PSMD11
• Gene ID (NCBI): 5717
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O00231
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The 26 S proteasome is a 2.5-MDa molecular machine that degrades ubiquitinated proteins in eukaryotic cells. It consists of a proteolytic core particle and two 19 S regulatory particles (RPs) composed of 6 ATPase (RPT) and 13 non-ATPase (RPN) subunits. PSMD11 gene encodes 19S proteasome subunit RPN6. Increased levels of PSMD11 and a corresponding increased assembly of the 26S/30S proteasome is correlated with high proteasome activity. In vitro ectopic expression of PSMD11 is sufficient to increase proteasome assembly and activity. Latest research has implicated PSMD11 in regulation of human embryonic stem cells function.