Tested Applications
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, Y79 cells |
| Positive IP detected in | HEK-293T cells |
| Positive IHC detected in | human kidney tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | Hela cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
15261-1-AP targets PSMD13 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag7257 Product name: Recombinant human PSMD13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC001100 Sequence: MKDVPGFLQQSQSSGPGQPAVWHRLEELYTKKLWHQLTLQVLDFVQDPCFAQGDGLIKLYENFISEFEHRVNPLSLVEIILHVVRQMTDPNVALTFLEKTREKVKSSDEAVILCKTAIGALKLNIGDLQVTKETIEDVEEMLNNLPGVTSVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVDIKDLPVSEQQERAFTLGLAGLLGEGVFNFGELLMHPVLESLRNTDRQWLIDTLYAFNSGNVERFQTLKTAWGQQPDLAANEAQLLRKIQLLCLMEMTFTRPANHRQLTFEEIAKSAKITVNEVELLVMKALSVGLVKGSIDEVDKRVHMTWVQPRVLDLQQIKGMKDRLEFWCTDVKSMEMLVEHQAHDILT Predict reactive species |
| Full Name | proteasome (prosome, macropain) 26S subunit, non-ATPase, 13 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 43 kDa |
| GenBank Accession Number | BC001100 |
| Gene Symbol | PSMD13 |
| Gene ID (NCBI) | 5719 |
| RRID | AB_2172977 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UNM6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| IHC protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| IP protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| WB protocol for PSMD13 antibody 15261-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
PSMD13 Polyclonal antibody (Cat# 15261-1-AP) has 7 total citations published in J Cell Biol, J Neurosci, J Biol Chem, Mamm Genome, J Biochem, Physiol Rep, and bioRxiv. Research fields span proteasome gene expression and transcription factor regulation, Leber Congenital Amaurosis and retinal disease, hepatocellular carcinoma prognosis and immunology, retinoid isomerase activity, and SKP2 ubiquitination in placental biology.
| Species | Application | Title |
|---|---|---|
J Cell Biol Proteasome gene expression is controlled by coordinated functions of multiple transcription factors | ||
J Neurosci Pharmacological Amelioration of Cone Survival and Vision in a Mouse Model for Leber Congenital Amaurosis.
| ||
J Biol Chem Rescue of enzymatic function for disease-associated RPE65s containing various missense mutations in non-active sites. | ||
Mamm Genome Comprehensive analysis reveals the prognostic and immunological role of PSMD13 in hepatocellular carcinoma | ||
J Biochem Temperature sensitive retinoid isomerase activity of RPE65 mutants associated with Leber congenital amaurosis. | ||
Physiol Rep Evidence that S-phase kinase associated protein 2 (SKP2) is ubiquitinated and degraded via a Rho-related BTB domain containing 1 (RhoBTB1) and Cullin-3 mechanism in placenta. |
