PSMD13 Monoclonal antibody

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  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 67807-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 67807-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 67807-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67807-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSMD13 antibody (67807-1-Ig, Clone: 2G10G6 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

PSMD13 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 67807-1-Ig

Clone No.2G10G6

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit, pig, chicken

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

HSPC027, p40.5, PSMD13, Rpn9, S11

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Tested Applications

• Positive WB detected in: HCT 116 cells, SMMC-7721 cells, HepG2 cells, HuH-7 cells, mouse liver tissue, NIH/3T3 cells, HSC-T6 cells, K-562 cells, Jurkat cells, HEK-293 cells, HeLa cells, L02 cells, A549 cells, Calu-3 cells, rat liver tissue, rabbit liver tissue, pig liver, chicken liver
• Positive IHC detected in: human pancreas cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67807-1-Ig targets PSMD13 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat, rabbit, pig, chicken samples.

• Tested Reactivity: Human, mouse, rat, rabbit, pig, chicken
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7857

  Product name: Recombinant human PSMD13 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-376 aa of BC001100

  Sequence: MKDVPGFLQQSQSSGPGQPAVWHRLEELYTKKLWHQLTLQVLDFVQDPCFAQGDGLIKLYENFISEFEHRVNPLSLVEIILHVVRQMTDPNVALTFLEKTREKVKSSDEAVILCKTAIGALKLNIGDLQVTKETIEDVEEMLNNLPGVTSVHSRFYDLSSKYYQTIGNHASYYKDALRFLGCVDIKDLPVSEQQERAFTLGLAGLLGEGVFNFGELLMHPVLESLRNTDRQWLIDTLYAFNSGNVERFQTLKTAWGQQPDLAANEAQLLRKIQLLCLMEMTFTRPANHRQLTFEEIAKSAKITVNEVELLVMKALSVGLVKGSIDEVDKRVHMTWVQPRVLDLQQIKGMKDRLEFWCTDVKSMEMLVEHQAHDILT

• Full Name: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC001100
• Gene Symbol: PSMD13
• Gene ID (NCBI): 5719
• RRID: AB_2918570
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9UNM6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.