CoraLite®594-conjugated PSMD4 Monoclonal antibody

PSMD4 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3G1A1

Cat no : CL594-66179

Synonyms

AF, AF 1, Antisecretory factor 1, ASF, MCB1, PSMD4, pUB R5, Rpn10, S5A



Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66179-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

CL594-66179 targets PSMD4 in IF applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMD4 fusion protein Ag7010
Full Name proteasome (prosome, macropain) 26S subunit, non-ATPase, 4
Calculated molecular weight 41 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002365
Gene symbol PSMD4
Gene ID (NCBI) 5710
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths593 nm/614 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMD4, also named as MCB1, AF or ASF, belongs to the proteasome subunit S5A family. It binds and presumably selects ubiquitin-conjugates for destruction. PSMD4 displays selectivity for longer polyubiquitin chains. It modulates intestinal fluid secretion. PSMD4 is exposed on the sperm acrosomal surface, a notion that was further supported by the binding of non-cell permeant, biotinylated proteasomal inhibitor ZL3VS to the sperm acrosome. It is involved in the sperm-ZP penetration during fertilization. (PMID: 20526895) This antibody is specific to PSMD4.

Protocols

Product Specific Protocols
IF protocol for CL594 PSMD4 antibody CL594-66179Download protocol
Standard Protocols
Click here to view our Standard Protocols