PSME3 Polyclonal antibody

• Featured Product
• KD/KO Validated

PSME3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

8

Reviews (1)

Cat no : 14907-1-AP

Synonyms

REG GAMMA, PA28gamma, PA28g, PA28 gamma, Ki nuclear autoantigen

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Validation Data Gallery

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  WB analysis using 14907-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14907-1-AP (PSME3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 14907-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 14907-1-AP (PSME3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14907-1-AP

  WB result of PSME3 antibody (14907-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSME3 transfected HepG2 cells.

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  WB analysis of mouse spleen using 14907-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14907-1-AP (PSME3 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 14907-1-AP

  IP result of anti-PSME3 (IP:14907-1-AP, 3ug; Detection:14907-1-AP 1:500) with COLO 320 cells lysate 2000ug.

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  IHC staining of human kidney using 14907-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14907-1-AP (PSME3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14907-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14907-1-AP (PSME3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 14907-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSME3 antibody 14907-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, mouse spleen tissue, HepG2 cells, Caco-2 cells, HCT 116 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 2 publications below

Product Information

14907-1-AP targets PSME3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSME3 fusion protein Ag6702
• Full Name: proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC001423
• Gene Symbol: PSME3
• Gene ID (NCBI): 10197
• RRID: AB_2171098
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSME3 gene encodes proteasome activator complex subunit 3, which is also called REG-gamma or PA28-gamma. REG-gamma activates the trypsin-like catalytic subunit of the proteasome thus is a proteasome regulator. MDM2-TP53 interaction which promotes ubiquitination- and MDM2-dependent proteasomal degradation of TP53, may be promoted by REG-gamma resulting in inhibited apoptosis after DNA damage. REG-gamma may also be involved in cell cycle regulation.

Publications

Species	Application	Title
human	WB,IHC	Cancer Lett; Proteasome activator subunit 3 promotes pancreatic cancer growth via c-Myc-glycolysis signaling axis.; Authors - Jiefang Guo
human	IHC	EBioMedicine; Indirubin-3'-monoxime acts as proteasome inhibitor: Therapeutic application in multiple myeloma.; Authors - Zhen Yu ; KD Validated
human	WB	Retrovirology; Comparative host protein interactions with HTLV-1 p30 and HTLV-2 p28: insights into difference in pathobiology of human retroviruses.; Authors - Doueiri Rami R
human	WB,IHC	PeerJ; REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma.; Authors - Zhiqiang Yin ; KD Validated
	IF	Nat Aging; Cold temperature extends longevity and prevents disease-related protein aggregation through PA28γ-induced proteasomes; Authors - Hyun Ju Lee
human	WB,IHC	Am J Transl Res; The oncogenic role of REG γ is exerted by activating the Wnt/β-catenin signaling pathway in osteosarcoma; Authors - Zhiqiang Yin ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Laetitia (Verified Customer) (03-21-2024)

Finding antibodies specific to avian proteins is very difficult. I have tried different antibodies from different companies so far. This antibody is by far the best (cost efficient as well)

• Applications: Western Blot, Immunofluorescence
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Epithelial chicken cells