PSMF1 Polyclonal antibody

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• KD/KO Validated

PSMF1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12941-1-AP

Synonyms

hPI31, PI31, PSMF1

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Validation Data Gallery

• 

  WB analysis using 12941-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12941-1-AP

  WB result of PSMF1 antibody (12941-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMF1 transfected HEK-293 cells.

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  WB analysis of human kidney using 12941-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12941-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12941-1-AP (PSMF1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, human kidney tissue, human heart tissue, Jurkat cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12941-1-AP targets PSMF1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PSMF1 fusion protein Ag4011
• Full Name: proteasome (prosome, macropain) inhibitor subunit 1 (PI31)
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC029836
• Gene Symbol: PSMF1
• Gene ID (NCBI): 9491
• RRID: AB_2268855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PSMF1 is also named as PI31(proteasome (prosome, macropain) inhibitor subunit 1) and belongs to proteasome inhibitor PI31 family. PI31, which localizes at the nuclear envelope/endoplasmic reticulum membrane, acts as a selective modulator of the proteasome-mediated steps in MHC class I antigen processing(PMID:18495667). PI31 does not inhibit cell-surface transport of glycoproteins in general and it may serve to control immunoproteasome formation and may thereby maintain an intracellular balance between constitutive and immunoproteasomes(PMID:12374861).

Publications

Species	Application	Title
human	WB	Mol Cell Biol; Interactome analysis reveals a novel role for RAD6 in the regulation of proteasome activity and localization in response to DNA damage.; Authors - Hongli An