PTEN Polyclonal antibody

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  WB analysis using 10047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293T using 10047-1-AP

  WB result of PTEN antibody (10047-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HEK-293T cells.

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  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293T using 10047-1-AP

  IP result of anti-PTEN (IP:10047-1-AP, 4ug; Detection:10047-1-AP 1:3000) with HEK-293T cells lysate 2040 ug.

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  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

PTEN Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10047-1-AP

Publications(18)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

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Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, HEK-293T cells, DU 145 cells
• Positive IP detected in: HEK-293T cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

10047-1-AP targets PTEN in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTEN fusion protein Ag0007
• Full Name: phosphatase and tensin homolog
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC005821
• Gene Symbol: PTEN
• Gene ID (NCBI): 5728
• RRID: AB_2174343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P60484
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa.

Publications

Species	Application	Title
human,mouse	WB,IHC,IF	Oxid Med Cell Longev; Knockdown of miR-214 Alleviates Renal Interstitial Fibrosis by Targeting the Regulation of the PTEN/PI3K/AKT Signalling Pathway; Authors - DongHua Hou
human	WB	Redox Biol; Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.; Authors - Tao Zeng
rat	WB,IHC	Cancers (Basel); Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1; Authors - Tiantian Qiu
human	WB	Biol Direct; M2 macrophage-derived exosomes promote angiogenesis and improve cardiac function after myocardial infarction; Authors - Hongzhou Guo
human	WB	J Cell Mol Med; Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN-PI3K-AKT signalling pathway in gastric cancer.; Authors - Shusheng Wu
human,mouse	WB,IF	iScience; Smurf1 silencing restores PTEN expression that ameliorates progression of human glioblastoma and sensitizes tumor cells to mTORC1/C2 inhibitor Torin1.; Authors - Qin Xia ; KD Validated