PTEN Polyclonal antibody

• Featured Product
• KD/KO Validated

PTEN Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 10047-1-AP

Synonyms

10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

---

Validation Data Gallery

• 

  WB analysis of HeLa using 10047-1-AP

  WB result of PTEN antibody (10047-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTEN transfected HeLa cells.

• 

  WB analysis using 10047-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 10047-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10047-1-AP (PTEN antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 10047-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10047-1-AP (PTEN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: MCF-7 cells, HeLa cells, DU 145 cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Product Information

10047-1-AP targets PTEN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTEN fusion protein Ag0007
• Full Name: phosphatase and tensin homolog
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 55 kDa
• GenBank accession number: BC005821
• Gene symbol: PTEN
• Gene ID (NCBI): 5728
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.

Publications

Species	Application	Title
human	WB	Redox Biol; Impairment of Akt activity by CYP2E1 mediated oxidative stress is involved in chronic ethanol-induced fatty liver.; Authors - Tao Zeng
rat	WB,IHC	Cancers (Basel); Astragaloside IV Inhibits the Proliferation of Human Uterine Leiomyomas by Targeting IDO1; Authors - Tiantian Qiu
human	WB,IHC	J Biomol Struct Dyn; Molecular dynamic simulation and functional analysis of pathogenic PTEN mutations in glioblastoma; Authors - Vildan Bozok Cetintas
human	WB	J Cell Mol Med; Knockdown of CENPK inhibits cell growth and facilitates apoptosis via PTEN-PI3K-AKT signalling pathway in gastric cancer.; Authors - Shusheng Wu
human,mouse	WB,IF	iScience; Smurf1 silencing restores PTEN expression that ameliorates progression of human glioblastoma and sensitizes tumor cells to mTORC1/C2 inhibitor Torin1.; Authors - Qin Xia ; KD Validated
rat	WB	Front Pharmacol; Analysis of the absorbed constituents and mechanism of liquidambaris fructus extract on hepatocellular carcinoma; Authors - Shuai Wang