Validation Data Gallery
|Positive WB detected in||fetal human brain tissue, RAW 264.7 cells, DU 145 cells, HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells|
|Positive IHC detected in||human breast hyperplasia tissue, human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue, human prostate hyperplasia tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunohistochemistry (IHC)||IHC : 1:200-1:1200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
60300-1-Ig targets PTEN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Mouse / IgG2a|
|Immunogen||PTEN fusion protein Ag17274|
|Full Name||phosphatase and tensin homolog|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||55 kDa|
|GenBank accession number||BC005821|
|Gene ID (NCBI)||5728|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.
Eur J Obstet Gynecol Reprod Biol
Comprehensive assessment the expression of core elements related to IGFIR/PI3K pathway in granulosa cells of women with polycystic ovary syndrome.
Evid Based Complement Alternat Med
Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.
Cell Commun Signal
The Janus-faced role of TRPM2-S in retroperitoneal liposarcoma via increasing ROS levels
NMC Case Rep J
Pediatric Giant Cell Glioblastoma Presenting with Intracranial Dissemination at Diagnosis: A Case Report.
Cell Death Dis
PTEN alleviates maladaptive repair of renal tubular epithelial cells by restoring CHMP2A-mediated phagosome closure.
BMC Complement Med Ther
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Georgina (Verified Customer) (11-04-2021)
Good primary antibody for PTEN. There is some background staining, however the band for PTEN is clear.