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PTEN Monoclonal antibody

PTEN Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse







Cat no : 60300-1-Ig


10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Tested Applications

Positive WB detected inDU 145 cells, fetal human brain tissue, HeLa cells, HEK-293 cells, MCF-7 cells, NIH/3T3 cells, HepG2 cells
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:200-1:1200
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60300-1-Ig targets PTEN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PTEN fusion protein Ag17274
Full Name phosphatase and tensin homolog
Calculated Molecular Weight 47 kDa
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC005821
Gene Symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.


Product Specific Protocols
WB protocol for PTEN antibody 60300-1-IgDownload protocol
IHC protocol for PTEN antibody 60300-1-IgDownload protocol
IF protocol for PTEN antibody 60300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Mol Cell

Fumarate inhibits PTEN to promote tumorigenesis and therapeutic resistance of type2 papillary renal cell carcinoma.

Authors - Xin Ge


Long noncoding RNA TSLNC8 is a tumor suppressor that inactivates the interleukin-6/STAT3 signaling pathway.

Authors - Jiwei Zhang


Cyclin G1-mediated epithelial-mesenchymal transition via phosphoinositide 3-kinase/Akt signaling facilitates liver cancer progression.

Authors - Wen Wen W

Nat Commun

The deubiquitylase OTUD3 stabilizes GRP78 and promotes lung tumorigenesis.

Authors - Tongde Du

Cancer Res

Targeting USP7 identifies a metastasis-competent state within bone marrow-resident melanoma CTCs.

Authors - Monika Vishnoi

J Transl Med

HNRNPA2B1-mediated m6A modification of lncRNA MEG3 facilitates tumorigenesis and metastasis of non-small cell lung cancer by regulating miR-21-5p/PTEN axis

Authors - Ke Li


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Georgina (Verified Customer) (11-04-2021)

Good primary antibody for PTEN. There is some background staining, however the band for PTEN is clear.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000