• Featured Product
  • KD/KO Validated

PTEN Monoclonal antibody

PTEN Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a


human, mouse







Cat no : 60300-1-Ig


10q23del, BZS, MHAM, MMAC1, phosphatase and tensin homolog, PTEN, PTEN1, TEP1

Tested Applications

Positive WB detected infetal human brain tissue, RAW 264.7 cells, DU 145 cells, HeLa cells, HEK-293 cells, MCF-7 cells, HepG2 cells
Positive IHC detected inhuman breast hyperplasia tissue, human breast cancer tissue, human endometrial cancer tissue, human lung cancer tissue, human prostate hyperplasia tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:1200
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

60300-1-Ig targets PTEN in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen PTEN fusion protein Ag17274
Full Name phosphatase and tensin homolog
Calculated molecular weight 47 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005821
Gene symbol PTEN
Gene ID (NCBI) 5728
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PTEN (also designated MMAC1), products of tumor suppressor genes, are found deleted in most human gliomas. The PTEN genes are also mutated in many other tumors, such as brain, breast, kidney and prostate cancers. PTEN is a protein tyrosine phosphatase that may terminate the signaling transduction pathways mediated by PI 3-kinase/Akt. PTEN has an apparent molecular weight of 55 kDa and it is located in the cytosol.


Product Specific Protocols
WB protocol for PTEN antibody 60300-1-IgDownload protocol
IHC protocol for PTEN antibody 60300-1-IgDownload protocol
IF protocol for PTEN antibody 60300-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Eur J Obstet Gynecol Reprod Biol

Comprehensive assessment the expression of core elements related to IGFIR/PI3K pathway in granulosa cells of women with polycystic ovary syndrome.

Authors - Tingting He

Evid Based Complement Alternat Med

Tetrandrine-Induced Autophagy in MDA-MB-231 Triple-Negative Breast Cancer Cell through the Inhibition of PI3K/AKT/mTOR Signaling.

Authors - Yubo Guo

Cell Commun Signal

The Janus-faced role of TRPM2-S in retroperitoneal liposarcoma via increasing ROS levels

Authors - Xiangji Li

NMC Case Rep J

Pediatric Giant Cell Glioblastoma Presenting with Intracranial Dissemination at Diagnosis: A Case Report.

Authors - Takamasa Kinoshita

Cell Death Dis

PTEN alleviates maladaptive repair of renal tubular epithelial cells by restoring CHMP2A-mediated phagosome closure.

Authors - Huizhen Wang
  • KD Validated

BMC Complement Med Ther

PMID: 32066429

Authors - Li Han


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Georgina (Verified Customer) (11-04-2021)

Good primary antibody for PTEN. There is some background staining, however the band for PTEN is clear.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000