PTGES2 Recombinant monoclonal antibody

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  WB analysis using 85419-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85419-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85419-4-RR (PTGES2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 85419-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85419-4-RR (PTGES2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85419-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85419-4-RR (PTGES2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 85419-4-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85419-4-RR (PTGES2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 85419-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of HeLa using 85419-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PTGES2 antibody (85419-4-RR, Clone: 242930B4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85419-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85419-4-RR against Human PTGES2 were performed. The affinity constant is 0.973 nM.

PTGES2 Uni-rAb^® Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 85419-4-RR

Clone No.242930B4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

242930B4, C9orf15, EC:5.3.99.3, GBF1, Membrane-associated prostaglandin E synthase-2

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, HepG2 cells, HEK-293 cells, Jurkat cells, rat heart tissue, pig heart tissue
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85419-4-RR targets PTGES2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1329

  Product name: Recombinant human PTGES2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-377 aa of BC011613

  Sequence: MDPAARVVRALWPGGCALAWRLGGRPQPLLPTQSRAGFAGAAGGPSPVAAARKGSPRLLGAAALALGGALGLYHTARWHLRAQDLHAERSAAQLSLSSRLQLTLYQYKTCPFCSKVRAFLDFHALPYQVVEVNPVRRAEIKFSSYRKVPILVAQEGESSQQLNDSSVIISALKTYLVSGQPLEEIITYYPAMKAVNEQGKEVTEFGNKYWLMLNEKEAQQVYGGKEARTEEMKWRQWADDWLVHLISPNVYRTPTEALASFDYIVREGKFGAVEGAVAKYMGAAAMYLISKRLKSRHRLQDNVREDLYEAADKWVAAVGKDRPFMGGQKPNLADLAVYGVLRVMEGLDAFDDLMQHTHIQPWYLRVERAITEASPAH

• Full Name: prostaglandin E synthase 2
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 30-35 kDa
• GenBank Accession Number: BC011613
• Gene Symbol: PTGES2
• Gene ID (NCBI): 80142
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9H7Z7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTGES2 (Prostaglandin E synthase 2) catalyzes the conversion of unstable intermediate of prostaglandin E2 H2 (PGH2) into the more stable prostaglandin E2 (PGE2) form. Its predicted size is 42 kDa, but it was detected one band of 35 kDa in the prostate and two bands of 30 kDa, 50 kDa in liver by western blot (PMID:17566096). PTGES2 protein is a homotetramer, whereas two PTGES2 molecules each form a strongly interacting dimer(PMID:19268562).