PTGFR Polyclonal antibody

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• PTGFR Polyclonal antibody (21595-1-AP)

• 

  WB analysis using 21595-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21595-1-AP (PTGFR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of PC-3 using 21595-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using PTGFR antibody (21595-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

PTGFR Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 21595-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FP, PGF receptor, PGF2 alpha receptor, prostaglandin F receptor (FP), Prostanoid FP receptor

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Tested Applications

• Positive WB detected in: fetal human brain tissue, mouse brain tissue, rat brain tissue
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21595-1-AP targets PTGFR in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16173

  Product name: Recombinant human PTGFR protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 305-359 aa of BC112965

  Sequence: ILLRKAVLKNLYKLASQCCGVHVISLHIWELSSIKNSLKVAAISESPVAEKSAST

• Full Name: prostaglandin F receptor (FP)
• Calculated Molecular Weight: 359 aa, 40 kDa
• Observed Molecular Weight: 30-40 kDa
• GenBank Accession Number: BC112965
• Gene Symbol: PTGFR
• Gene ID (NCBI): 5737
• RRID: AB_3742046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43088
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Prostanoid FP receptor(PTGFR) is a member of the G-protein coupled receptor family and is involved in several physiologic processes. PTGFR is a receptor for prostaglandin F2-alpha (PGF2-alpha), which is known to be a potent luteolytic agent, and may also be involved in modulating intraocular pressure and smooth muscle contraction in the uterus(PMID: 9235889).