CD315/PTGFRN Polyclonal antibody, PBS Only

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• CD315/PTGFRN Polyclonal antibody, PBS Only (33863-1-PBS)

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  WB analysis using 33863-1-AP (same clone as 33863-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 33863-1-AP (CD315/PTGFRN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33863-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 33863-1-AP (same clone as 33863-1-PBS)

  IP result of anti-CD315/PTGFRN (IP:33863-1-AP, 4ug; Detection:33863-1-AP 1:300) with HeLa cells lysate 1480 ug. This data was developed using the same antibody clone with 33863-1-PBS in a different storage buffer formulation.

CD315/PTGFRN Polyclonal Antibody for WB, IP, Indirect ELISA

Cat No. 33863-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, Indirect ELISA

Synonyms

PTGFRN, CD315, EWIF, FPRP, KIAA1436

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Product Information

33863-1-PBS targets CD315/PTGFRN in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Eg5010

  Product name: Recombinant Human PTGFRN/CD315 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 26-832 aa of BC152454

  Sequence: VPTATLVRVVGTELVIPCNVSDYDGPSEQNFDWSFSSLGSSFVELASTWEVGFPAQLYQERLQRGEILLRRTANDAVELHIKNVQPSDQGHYKCSTPSTDATVQGNYEDTVQVKVLADSLHVGPSARPPPSLSLREGEPFELRCTAASASPLHTHLALLWEVHRGPARRSVLALTHEGRFHPGLGYEQRYHSGDVRLDTVGSDAYRLSVSRALSADQGSYRCIVSEWIAEQGNWQEIQEKAVEVATVVIQPSVLRAAVPKNVSVAEGKELDLTCNITTDRADDVRPEVTWSFSRMPDSTLPGSRVLARLDRDSLVHSSPHVALSHVDARSYHLLVRDVSKENSGYYYCHVSLWAPGHNRSWHKVAEAVSSPAGVGVTWLEPDYQVYLNASKVPGFADDPTELACRVVDTKSGEANVRFTVSWYYRMNRRSDNVVTSELLAVMDGDWTLKYGERSKQRAQDGDFIFSKEHTDTFNFRIQRTTEEDRGNYYCVVSAWTKQRNNSWVKSKDVFSKPVNIFWALEDSVLVVKARQPKPFFAAGNTFEMTCKVSSKNIKSPRYSVLIMAEKPVGDLSSPNETKYIISLDQDSVVKLENWTDASRVDGVVLEKVQEDEFRYRMYQTQVSDAGLYRCMVTAWSPVRGSLWREAATSLSNPIEIDFQTSGPIFNASVHSDTPSVIRGDLIKLFCIITVEGAALDPDDMAFDVSWFAVHSFGLDKAPVLLSSLDRKGIVTTSRRDWKSDLSLERVSVLEFLLQVHGSEDQDFGNYYCSVTPWVKSPTGSWQKEAEIHSKPVFITVKMDVLNAFKYP

• Full Name: prostaglandin F2 receptor negative regulator
• Calculated Molecular Weight: 879 aa, 99 kDa
• Observed Molecular Weight: 100-130 kDa
• GenBank Accession Number: BC152454
• Gene Symbol: PTGFRN
• Gene ID (NCBI): 5738
• RRID: AB_3743099
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q9P2B2
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTGFRN (Prostaglandin F2 Receptor Negative Regulator), also known as CD315, is a trans-membrane protein that modulates prostaglandin F2α signaling and links the tetraspanin web to the cytoskeleton, it is now recognized as a driver of metastasis; high expression correlates with aggressive proliferation, migration, and spheroid growth in epidermoid carcinoma, medulloblastoma, and mesothelioma, while silencing PTGFRN impairs these traits and disrupts autophagy (PMID: 38924562). The calculated value of PTGFRN is 98 kDa. It has multiple glycosylation sites and its molecular weight after glycosylation is approximately 100 - 130 kDa.