PTGIS Recombinant monoclonal antibody

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  WB analysis using 85810-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85810-1-RR (PTGIS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 85810-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PTGIS antibody (85810-1-RR, Clone: 243128C2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85810-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85810-1-RR against Human PTGIS were performed. The affinity constant is below 1 pM.

PTGIS Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 85810-1-RR

Clone No.243128C2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

CYP8, CYP8A1, EC:4.2.1.152, EC:5.3.99.4, Hydroperoxy icosatetraenoate dehydratase

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Tested Applications

• Positive WB detected in: SKOV-3 cells, mouse testis tissue
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85810-1-RR targets PTGIS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag25817

  Product name: Recombinant human PTGIS protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 100-285 aa of BC101809

  Sequence: AIFLMERIFDVQLPHYSPSDEKARMKLTLLHRELQALTEAMYTNLHAVLLGDATEAGSGWHEMGLLDFSYSFLLRAGYLTLYGIEALPRTHESQAQDRVHSADVFHTFRQLDRLLPKLARGSLSVGDKDHMCSVKSRLWKLLSPARLARRAHRSKWLESYLLHLEEMGVSEEMQARALVLQLWATQ

• Full Name: prostaglandin I2 (prostacyclin) synthase
• Calculated Molecular Weight: 500 aa, 57 kDa
• Observed Molecular Weight: 50-57 kDa
• GenBank Accession Number: BC101809
• Gene Symbol: PTGIS
• Gene ID (NCBI): 5740
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16647
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.