PTH1R Polyclonal antibody

PTH1R Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24275-1-AP

Synonyms

parathyroid hormone 1 receptor, PFE, PTH/PTHr receptor, PTH/PTHrP type I receptor, PTH1 receptor, PTH1R, PTHR, PTHR1

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Validation Data Gallery

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  WB analysis of U2OS using 24275-1-AP

  U2OS cells were subjected to SDS PAGE followed by western blot with 24275-1-AP (PTH1R antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 24275-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24275-1-AP (PTH1R antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 24275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 24275-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 24275-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 24275-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 24275-1-AP (PTH1R antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: U2OS cells, HepG2 cells, mouse liver tissue
• Positive IHC detected in: mouse kidney tissue, human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

24275-1-AP targets PTH1R in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTH1R fusion protein Ag17577
• Full Name: parathyroid hormone 1 receptor
• Calculated Molecular Weight: 593 aa, 66 kDa
• Observed Molecular Weight: 70-80 kDa
• GenBank Accession Number: BC110388
• Gene Symbol: PTH1R
• Gene ID (NCBI): 5745
• RRID: AB_2879465
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTH1R is a receptor for parathyroid hormone and parathyroid hormone-related peptide (PTHrP). PTH1R is a glycoprotein with seven transmembrane domains and it has an extracellular N-terminus region and an intracellular C-terminus region. The activity of PTH1R is mediated by G proteins which activate adenylyl cyclase. It has been reported that PTHrP and PTH1R are associated with the differentiation of bone and cartilage in the developing stages. PTH1R has several isoforms with 66/59 kDa and PTH1R can form a 120 kDa dimer in the non-reducing condition. 24275-1-AP antibody detects 80 kDa protein which is similar to the results reported in publications.(PMID: 19507249, 27160269)