PRL3 Polyclonal antibody

PRL3 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 15186-1-AP

Synonyms

PTP4A3, Protein-tyrosine phosphatase 4a3, PRL-R, PRL-3, PRL R

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Validation Data Gallery

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  WB analysis using 15186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15186-1-AP (PRL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse heart tissue, rat heart tissue
• Positive IHC detected in: human colon cancer tissue, human oesophagus cancer tissue, human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

15186-1-AP targets PRL3 in IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PRL3 fusion protein Ag7249
• Full Name: protein tyrosine phosphatase type IVA, member 3
• Calculated Molecular Weight: 20 kDa
• Observed Molecular Weight: 20-28 kDa
• GenBank Accession Number: BC003105
• Gene Symbol: PTP4A3
• Gene ID (NCBI): 11156
• RRID: AB_2878115
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Phosphatase of regenerating liver-3 (PRL-3; also known as PTP4A3), encodes a 22-kDa protein that represents a novel class of a superfamily of protein tyrosine phosphatases (PTPs) that are ubiquitously expressed in various tumors. PRL3 was associated with primary tumor growth, tumor reoccurrence, and therapy resistance in many human cancers. This protein was also suggested as a marker to predict the relapse of gastric cancer and invasive breast cancer. (PMID: 22469786, PMID: 30157875)

Publications

Species	Application	Title
mouse	IHC	J Pharmacol Exp Ther; KVX-053, a Protein Tyrosine Phosphatase 4A3 inhibitor, ameliorates SARS-CoV-2 Spike protein subunit 1 - induced acute lung injury in mice; Authors - Pavel A Solopov
human	IHC	Reprod Sci; PRL-3 Is Involved in Estrogen- and IL-6-Induced Migration of Endometrial Stromal Cells From Ectopic Endometrium.; Authors - Shifan Ren
human	IF	Mol Cancer; Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma; Authors - Jianbiao Zhou