PTPN1/PTP1B Recombinant antibody, PBS Only

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  WB analysis using 85905-4-RR (same clone as 85905-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85905-4-RR (PTPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85905-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85905-4-RR (same clone as 85905-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN1 antibody (85905-4-RR, Clone: 250215G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85905-4-PBS in a different storage buffer formulation.

PTPN1/PTP1B Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85905-4-PBS

Clone No.250215G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

PTP1B, PTPN1, EC:3.1.3.48, Protein-tyrosine phosphatase 1B, PTP 1B

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Product Information

85905-4-PBS targets PTPN1/PTP1B in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PTPN1/PTP1B fusion protein Ag1878
• Full Name: protein tyrosine phosphatase, non-receptor type 1
• Calculated Molecular Weight: 435 aa, 50 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC015660
• Gene Symbol: PTP1B
• Gene ID (NCBI): 5770
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P18031
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).