• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of various lysates using PTPN1 Recombinant antibody (85905-4-RR)

    WB analysis using 85905-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85905-4-RR (PTPN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PTPN1 Recombinant antibody (85905-4-RR)

    IF Staining of HeLa using 85905-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PTPN1 antibody (85905-4-RR, Clone: 250215G6 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

PTPN1/PTP1B Recombinant antibody

PTPN1/PTP1B Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85905-4-RR
Clone No.250215G6

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

PTP1B, PTPN1, EC:3.1.3.48, Protein-tyrosine phosphatase 1B, PTP 1B

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, Jurkat cells, THP-1 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85905-4-RR targets PTPN1/PTP1B in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PTPN1/PTP1B fusion protein Ag1878 Predict reactive species
Full Name protein tyrosine phosphatase, non-receptor type 1
Calculated Molecular Weight 435 aa, 50 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC015660
Gene Symbol PTP1B
Gene ID (NCBI) 5770
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP18031
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

Protocols

Product Specific Protocols
WB protocol for PTPN1/PTP1B antibody 85905-4-RRDownload protocol
IF protocol for PTPN1/PTP1B antibody 85905-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols