CoraLite®647-conjugated PTPN23 Polyclonal antibody

• 

  IF Staining of mouse heart using CL647-10472

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite®647 PTPN23 antibody (CL647-10472) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using CL647-10472

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®647 PTPN23 antibody (CL647-10472) at dilution of 1:200.

• Featured Product
• KD/KO Validated

PTPN23 Polyclonal Antibody for IF/ICC, IF-P

Cat No. CL647-10472

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, IF-P

Synonyms

EC:3.1.3.48, HD PTP, HDPTP, HD-PTP, His domain-containing protein tyrosine phosphatase

---

Tested Applications

• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-10472 targets PTPN23 in IF/ICC, IF-P applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0765

  Product name: Recombinant human PTPN23 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 420-619 aa of BC004881

  Sequence: QEKLHLRFCYEAVVRHVEQVLQRHGVPPPCKPLASASISQKNHLPQDSQDLVLGGDVPISSIQATIAKLSIRPPGGLESPVASLPGPAEPPGLPPASLPESTPIPSSSPPPLSSPLPEAPQPKEEPPVPEAPSSGPPSSSLELLASLTPEAFSLDSSLRGKQRMSKHNFLQAHNGQGLRATRPSDDPLSLLDPLWTLNKT

• Full Name: protein tyrosine phosphatase, non-receptor type 23
• Calculated Molecular Weight: 179 kDa
• Observed Molecular Weight: 179 kDa
• GenBank Accession Number: BC004881
• Gene Symbol: PTPN23
• Gene ID (NCBI): 25930
• RRID: AB_3084801
• Conjugate: CoraLite®647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H3S7
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PTPN23(Tyrosine-protein phosphatase non-receptor type 23) is also named as HD-PTP,PTP-TD14 and KIAA1471.It encodes a 185-kDa protein which is expressed in human endothelial fromthe umbilical cord and in human Kaposi-spindle cells(PMID:16408268).PTPN23 is a non-transmembrane protein tyrosine phosphatase which contributes to the signal transduction pathways involved in the regulation of cell migration and invasion(PMID:22510412).