PTPN7 Polyclonal antibody, PBS Only

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• PTPN7 Polyclonal antibody, PBS Only (15286-1-PBS)

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  WB analysis using 15286-1-AP (same clone as 15286-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

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  WB analysis of Raji using 15286-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15286-1-AP (same clone as 15286-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 15286-1-AP (same clone as 15286-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 15286-1-AP (PTPN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lymphoma using 15286-1-AP (same clone as 15286-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 15286-1-AP (PTPN7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15286-1-PBS in a different storage buffer formulation.

PTPN7 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15286-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

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Product Information

15286-1-PBS targets PTPN7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7453

  Product name: Recombinant human PTPN7 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-399 aa of BC001746

  Sequence: MGASFWPIRQAREQQRRALSFRQTSWLSEPPLGPAPHLSMVQAHGGRSRAQPLTLSLGAAMTQPPPEKTPAKKHVRLQERRGSNVALMLDVRSLGAVEPICSVNTPREVTLHFLRTAGHPLTRWALQRQPPSPKQLEEEFLKIPSNFVSPEDLDIPGHASKDRYKTILPNPQSRVCLGRAQSQEDGDYINANYIRGYDGKEKVYIATQGPMPNTVSDFWEMVWQEEVSLIVMLTQLREGKEKCVHYWPTEEETYGPFQIRIQDMKECPEYTVRQLTIQYQEERRSVKHILFSAWPDHQTPESAGPLLRLVAEVEESPETAAHPGPIVVHCSAGIGRTGCFIATRIGCQQLKARGEVDILGIVCQLRLDRGGMIQTAEQYQFLHHTLALYAGQLPEEPSP

• Full Name: protein tyrosine phosphatase, non-receptor type 7
• Calculated Molecular Weight: 41 kDa
• Observed Molecular Weight: 38-40 kDa
• GenBank Accession Number: BC001746
• Gene Symbol: PTPN7
• Gene ID (NCBI): 5778
• RRID: AB_2173831
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35236
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

PTPN7, also known as haematopoietic PTP (HePTP), belongs to the R7 family of protein tyrosine phosphatases (PTPs) which all contain a conserved 16 amino acid sequence at their N-termini (PMID: 9857190). PTPN7, via negative regulation of MAPK signalling, plays a key role in T-cell and B-cell development and is the only known PTP whose expression in T-lymphocytes is induced by IL-2 (PMID: 15980098). PTPN7 is a 38 kDa non-receptor PTP expressed predominately in leukocytes, spleen and thymus (PMID: 1530918, 24265715, 19636879).