PTPRCAP Polyclonal antibody

PTPRCAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 28286-1-AP

Synonyms

CD45 AP, CD45 associated protein, LPAP, PTPRC associated protein, PTPRCAP

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Validation Data Gallery

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  WB analysis of Raji using 28286-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 28286-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 28286-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 28286-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 28286-1-AP (PTPRCAP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human spleen using 28286-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 28286-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28286-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28286-1-AP (PTPRCAP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Jurkat cells, K-562 cells, Raji cells
• Positive IHC detected in: human spleen tissue, human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

28286-1-AP targets PTPRCAP in WB, IHC, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPRCAP fusion protein Ag27236
• Full Name: protein tyrosine phosphatase, receptor type, C-associated protein
• Calculated Molecular Weight: 206 aa, 21 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC113859
• Gene Symbol: PTPRCAP
• Gene ID (NCBI): 5790
• RRID: AB_2881105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Discov Oncol; New immunological potential markers for triple negative breast cancer: IL18R1, CD53, TRIM, Jaw1, LTB, PTPRCAP.; Authors - Paolo Marchetti