PTPRE Polyclonal antibody

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PTPRE Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13922-1-AP Copied!

Publications (3)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

EC:3.1.3.48, HPTPE, Protein-tyrosine phosphatase epsilon, PTPE, R PTP EPSILON

• 

  WB analysis of HL-60 using 13922-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 13922-1-AP (PTPRE antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 13922-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13922-1-AP (PTPRE Antibody) at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HL-60 cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13922-1-AP targets PTPRE in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4932

  Product name: Recombinant human PTPRE protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 351-700 aa of BC050062

  Sequence: MMAMMHAEQKVDVFEFVSRIRNQRPQMVQTDMQYTFIYQALLEYYLYGDTELDVSSLEKHLQTMHGTTTHFDKIGLEEEFRKLTNVRIMKENMRTGNLPANMKKARVIQIIPYDFNRVILSMKRGQEYTDYINASFIDGYRQKDYFIATQGPLAHTVEDFWRMIWEWKSHTIVMLTEVQEREQDKCYQYWPTEGSVTHGEITIEIKNDTLSEAISIRDFLVTLNQPQARQEEQVRVVRQFHFHGWPEIGIPAEGKGMIDLIAAVQKQQQQTGNHPITVHCSAGAGRTGTFIALSNILERVKAEGLLDVFQAVKSLRLQRPHMVQTLEQYEFCYKVVQDFIDIFSDYANFK

• Full Name: protein tyrosine phosphatase, receptor type, E
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC050062
• Gene Symbol: PTPRE
• Gene ID (NCBI): 5791
• RRID: AB_2174672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P23469
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The PTPRE antibody (Cat# 13922-1-AP) has 3 total citations published in Biomedical Pharmacotherapy, Clinical Epigenetics, and Frontiers in Oncology. The research fields span hepatocellular carcinoma tumor progression and sorafenib resistance, TET2-mediated ECM1 hypomethylation in proliferative diabetic retinopathy neovascularization, and miR-631 inhibition of intrahepatic metastasis in HCC. Applications include IHC and WB in human samples.

Automatically generated from the 3 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Biomed Pharmacother; Inhibition of PTPRE suppresses tumor progression and improves sorafenib response in hepatocellular carcinoma; Authors - Renshun Dong
human	WB	Clin Epigenetics; TET2-mediated ECM1 hypomethylation promotes the neovascularization in active proliferative diabetic retinopathy; Authors - Chunyang Cai
human	WB	Front Oncol; miR-631 Inhibits Intrahepatic Metastasis of Hepatocellular Carcinoma by Targeting PTPRE.; Authors - Bingqing Chen ; KD Validated