PTPRR Polyclonal antibody

PTPRR Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17937-1-AP

Synonyms

Ch 1PTPase, EC PTP, ECPTP, NC PTPCOM1, PCPTP1, PTP SL, PTPBR7, PTPRQ, PTPRR, R PTP R

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Validation Data Gallery

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  WB analysis using 17937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17937-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17937-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 17937-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, COLO 320 cells, SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17937-1-AP targets PTPRR in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTPRR fusion protein Ag12145
• Full Name: protein tyrosine phosphatase, receptor type, R
• Calculated Molecular Weight: 657 aa, 74 kDa
• Observed Molecular Weight: 32 kDa, 47 kDa, 65 kDa
• GenBank Accession Number: BC110900
• Gene Symbol: PTPRR
• Gene ID (NCBI): 5801
• RRID: AB_10694430
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PTPRR(protein tyrosine phosphatase, receptor type, R) is also named as PCPTP1, PTP-SL, PTPBR7 and belongs to the protein-tyrosine phosphatase family. It sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. It has four isoforms that they have molecular weights of 72, 60, 42 and 37 kDa, respectively, and differ in the length of their N-terminal part which result in distinct subcellular localizations. All PTPRR isoforms are found to be short-lived proteins, with half-lives of around 3-5 hours, and are constitutively phosphorylated on one or two of the previously identified protein kinase A and MAP kinase target sites.

Publications

Species	Application	Title
human	IHC	Mol Cancer; Multi-cohort validation of Ascore: an anoikis-based prognostic signature for predicting disease progression and immunotherapy response in bladder cancer; Authors - Tianlei Xie
mouse	WB	J Affect Disord; PTPRR regulates ERK dephosphorylation in depression mice model.; Authors - Xinrong Li
human	WB	BMC Cancer; Androgen-regulation of the protein tyrosine phosphatase PTPRR activates ERK1/2 signalling in prostate cancer cells.; Authors - Jennifer Munkley
mouse	WB,IF	Front Mol Neurosci; Downregulation of Protein Tyrosine Phosphatase Receptor Type R Accounts for the Progression of Hirschsprung Disease.; Authors - Jiao Tian