Tested Applications
| Positive WB detected in | HT-1080 cells, rat heart tissue, mouse heart tissue, mouse lung tissue |
| Positive IP detected in | HT-1080 cells |
| Positive IHC detected in | human normal colon, mouse brown adipose tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18892-1-AP targets PTRF in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag13486 Product name: Recombinant human PTRF protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-198 aa of BC008849 Sequence: MEDAAALELSSDEAVEVEEVIEESRAERIKRSGLRRVDDFKKAFSKEKMEKTKVRTRENLEKTRLKTKENLEKTRHTLEKRMNKLGTRLVPAERREKLKTSRDKLRKSFTPDHVVYARSKTAVYKVPPFTFHVKKIREGQVEVLKATEMVEVGADDDEGGAERGEAGDLRRGSSPDVHALLEITEESDAVLVDKSDSD Predict reactive species |
| Full Name | polymerase I and transcript release factor |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC008849 |
| Gene Symbol | PTRF |
| Gene ID (NCBI) | 284119 |
| RRID | AB_10596795 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6NZI2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transciption of RNA Pol I, which involves pausing of transcription by TTF1, and dissocaition of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55kDa. (PMID: 11139612 )
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTRF antibody 18892-1-AP | Download protocol |
| IHC protocol for PTRF antibody 18892-1-AP | Download protocol |
| IP protocol for PTRF antibody 18892-1-AP | Download protocol |
| WB protocol for PTRF antibody 18892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTRF Polyclonal Antibody (Cat# 18892-1-AP) has 48 citations across journals including Nature Communications, Cell Metabolism, Autophagy, ACS Nano, Theranostics, Science Advances, Developmental Cell, Cell Reports, EMBO Reports, Journal of Cell Biology, FASEB Journal, eLife, Oncotarget, and Neuro-Oncology. Research fields span caveolae biology, extracellular vesicles/exosomes, cancer (glioblastoma, prostate, melanoma, gastric, oral), cardiovascular disease and atherosclerosis, lipid metabolism, neurobiology, autophagy, ferroptosis, and immune signaling.
| Species | Application | Title |
|---|---|---|
J Extracell Vesicles Cavin-1/PTRF alters prostate cancer cell-derived extracellular vesicle content and internalization to attenuate extracellular vesicle-mediated osteoclastogenesis and osteoblast proliferation. | ||
Autophagy CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.
| ||
Nat Commun Mapping of endosomal proximity proteomes reveals Retromer as a hub for RAB GTPase regulation | ||
ACS Nano Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration. | ||
Theranostics PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma. |
Reviews
What customers say
Customers report this antibody performs reliably across multiple applications. One user found it works very well in Western Blot at 1:1000 in melanoma cell lines. Another rated it highly, noting it worked perfectly for immunofluorescence at 1:200 in several cell types (RPE-1, adipocytes, mesenchymal, HeLa, MOVAS), as well as for Western Blot at 1:2000 and immunoprecipitation. Overall, reviewers describe it as a great, versatile antibody with consistent results across WB, IF, and IP.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Celine (Verified Customer) (06-22-2023) | Works very well in Western Blot.
 |
Maria (Verified Customer) (01-27-2022) | Great antibody. It worked perfectly for IF 1:200 in several cell types (RPE-1, adipocytes, mesenchymal, HeLa or MOVAS), for WB (1:2000) and for IP.
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