PTRF Polyclonal antibody

• Featured Product
• KD/KO Validated

PTRF Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

32

Reviews (2)

Cat no : 18892-1-AP

Synonyms

FKSG13, Cavin-1, Cavin 1

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Validation Data Gallery

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  WB analysis using 18892-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18892-1-AP (PTRF antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 18892-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 18892-1-AP (PTRF Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HT-1080 using 18892-1-AP

  IP result of anti-PTRF (IP:18892-1-AP, 4ug; Detection:18892-1-AP 1:2000) with HT-1080 cells lysate 3600 ug.

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  IHC staining of mouse brown adipose using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 18892-1-AP (PTRF antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18892-1-AP (PTRF antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human normal colon using 18892-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 18892-1-AP (PTRF antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 18892-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PTRF antibody (18892-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

• Positive WB detected in: HT-1080 cells, rat heart tissue, mouse heart tissue, mouse lung tissue
• Positive IP detected in: HT-1080 cells
• Positive IHC detected in: human normal colon, mouse brown adipose tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 7 publications below
• WB: See 26 publications below
• IHC: See 6 publications below
• IF: See 9 publications below
• IP: See 2 publications below

Product Information

18892-1-AP targets PTRF in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PTRF fusion protein Ag13486
• Full Name: polymerase I and transcript release factor
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 50-55 kDa
• GenBank Accession Number: BC008849
• Gene Symbol: PTRF
• Gene ID (NCBI): 284119
• RRID: AB_10596795
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Polymerase I and transcript release factor(PTRF) is an essential factor in the biogenesis of caveolae, which involve in numerous process, such as signal transduction and membrane and lipid trafficking. PTRF can terminate transciption of RNA Pol I, which involves pausing of transcription by TTF1, and dissocaition of the transcription complex, release the complex from the template. The calculated molecular weight of PTRF is 43 kDa, but modified PTRF is about 50-55kDa. (PMID: 11139612 )

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
human	WB,IF	Sci Adv; Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.; Authors - Virginia Andrade ; KO Validated
human	WB,IF,IP	Dev Cell; Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2; Authors - Yeping Wu
human	WB,IHC	Autophagy; CAV1-CAVIN1-LC3B-mediated autophagy regulates high glucose-stimulated LDL transcytosis.; Authors - Xiangli Bai ; KD Validated
mouse	WB	Proc Natl Acad Sci U S A; Inducible Fgf13 ablation enhances caveolae-mediated cardioprotection during cardiac pressure overload.; Authors - Eric Q Wei
mouse	IF	Theranostics; PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma.; Authors - Eryan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Celine (Verified Customer) (06-22-2023)

Works very well in Western Blot.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Melanoma cell lines

PTRF Antibody Western Blot validation (1:1000 dilution) in Melanoma cell lines (Cat no:18892-1-AP)

FH

Maria (Verified Customer) (01-27-2022)

Great antibody. It worked perfectly for IF 1:200 in several cell types (RPE-1, adipocytes, mesenchymal, HeLa or MOVAS), for WB (1:2000) and for IP.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: RPE-1 /MSC/MOVAS