Tested Applications
| Positive WB detected in | A375 cells, HEK-293 cells, HeLa cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human testis tissue, human colon tissue, human liver tissue, mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, PC-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12575-1-AP targets PTTG1IP in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3250 Product name: Recombinant human PTTG1IP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-180 aa of BC012858 Sequence: MAPGVARGPTPYWRLRLGGAALLLLLIPVAAAQEPPGAACSQNTNKTCEECLKNVSCLWCNTNKACLDYPVTSVLPPASLCKLSSARWGVCWVNFEALIITMSVVGGTLLLGIAICCCCCCRRKRSRKPDRSEEKAMREREERRIRQEERRAEMKTRHDEIRKKYGLFKEENPYARFENN Predict reactive species |
| Full Name | pituitary tumor-transforming 1 interacting protein |
| Calculated Molecular Weight | 180 aa, 20 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC012858 |
| Gene Symbol | PTTG1IP |
| Gene ID (NCBI) | 754 |
| RRID | AB_10644145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P53801 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PTTG1IP antibody 12575-1-AP | Download protocol |
| IHC protocol for PTTG1IP antibody 12575-1-AP | Download protocol |
| IP protocol for PTTG1IP antibody 12575-1-AP | Download protocol |
| WB protocol for PTTG1IP antibody 12575-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PTTG1IP Polyclonal antibody (Cat# 12575-1-AP) has 4 citations published in Pharmacol Res, Front Biosci (Landmark Ed), Horm Metab Res, and Oncol Lett. The associated research fields include ferroptosis and TGF-β signaling in hepatocellular carcinoma, epithelial-mesenchymal transition (EMT) and DNA damage response in thyroid cancer, tumor suppression in pituitary tumors, and EMT protein expression in esophageal squamous cell carcinoma.
| Species | Application | Title |
|---|---|---|
Pharmacol Res PIAS3 promotes ferroptosis by regulating TXNIP via TGF-β signaling pathway in hepatocellular carcinoma | ||
Front Biosci (Landmark Ed) PTTG1IP Orchestrates Epithelial-Mesenchymal Transition and DNA Damage Response in Thyroid Cancer Progression.
| ||
Horm Metab Res MicroRNA-524-5p Functions as a Tumor Suppressor in a Human Pituitary Tumor-Derived Cell Line. | ||
Oncol Lett Expression of proteins associated with epithelial-mesenchymal transition in esophageal squamous cell carcinoma. |
