Tested Applications
| Positive WB detected in | A549 cells, LNCap cells, HeLa cells, HepG2 cells, K-562 cells, HSC-T6 cells, C2C12 cells, pig brain tissue, rabbit brain tissue |
| Positive IHC detected in | mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
61022-1-Ig targets PURB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat, rabbit samples.
| Tested Reactivity | human, mouse, rat, rabbit |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12816 Product name: Recombinant human PURB protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-312 aa of BC101735 Sequence: MADGDSGSERGGGGGPCGFQPASRGGGEQETQELASKRLDIQNKRFYLDVKQNAKGRFLKIAEVGAGGSKSRLTLSMAVAAEFRDSLGDFIEHYAQLGPSSPEQLAAGAEEGGGPRRALKSEFLVRENRKYYLDLKENQRGRFLRIRQTVNRGGGGFGAGPGPGGLQSGQTIALPAQGLIEFRDALAKLIDDYGGEDDELAGGPGGGAGGPGGGLYGELPEGTSITVDSKRFFFDVGCNKYGVFLRVSEVKPSYRNAITVPFKAWGKFGGAFCRYADEMKEIQERQRDKLYERRGGGSGGGEESEGEEVDED Predict reactive species |
| Full Name | purine-rich element binding protein B |
| Calculated Molecular Weight | 312 aa, 33 kDa |
| Observed Molecular Weight | 33-40 kDa |
| GenBank Accession Number | BC101735 |
| Gene Symbol | PURB |
| Gene ID (NCBI) | 5814 |
| RRID | AB_3743258 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96QR8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PURB (Purine-rich element-binding protein B) is a ubiquitously expressed DNA/RNA-binding protein. It localizes to both the nucleus and cytoplasm, reflecting its dual role in transcriptional regulation and RNA metabolism. Its primary function is to bind purine-rich sequences to control gene expression, cell cycle progression, and differentiation. The observed molecular weight of PURB by SDS-PAGE is 33-40 kDa, which is close to its predicted molecular weight of ~33 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PURB antibody 61022-1-Ig | Download protocol |
| WB protocol for PURB antibody 61022-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
