ASC/TMS1 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• ASC/TMS1 Polyclonal antibody (30641-1-AP)

• 

  WB analysis using 30641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30641-1-AP (PYCARD antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30641-1-AP (PYCARD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse colon using 30641-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 30641-1-AP (ASC/TMS1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 30641-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PYCARD antibody (30641-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

ASC/TMS1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 30641-1-AP

Publications(23)

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PYCARD, Apoptosis-associated speck-like protein containing a CARD, Asc, CARD5, masc

---

Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse thymus tissue, rat spleen tissue, rat thymus tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 19 publications below
• IF: See 5 publications below

Product Information

30641-1-AP targets ASC/TMS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag32519

  Product name: Recombinant mouse PYCARD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of NM_023258

  Sequence: MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS

• Full Name: PYD and CARD domain containing
• Calculated Molecular Weight: 21KD
• Observed Molecular Weight: 21-25 kDa
• GenBank Accession Number: NM_023258
• Gene Symbol: Pycard
• Gene ID (NCBI): 66824
• RRID: AB_3086379
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9EPB4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		J Agric Food Chem; Ginsenoside Ro Targets BCAT1/2 to Suppress Ferroptosis: A Novel Therapeutic Strategy for Ischemic Cardioprotection; Authors - Jincheng Liu
mouse	WB	Basic Res Cardiol; Renal denervation attenuates cardiac dysfunction in HFpEF by inhibiting the ATP-P2X7-NLRP3 inflammasome axis.; Authors - Zhuqing Li
mouse	WB	Phytomedicine; Jiangu formula modulates the gut microbiota structure and the NF-κB/NLRP3 signaling pathway to alleviate synovitis.; Authors - Shuying Li
mouse	WB	Front Pharmacol; Multitarget mechanisms of the herb pair Achyranthes bidentata and Paeonia lactiflora Pall. in ameliorating hypertensive cardiomyopathy: combining network pharmacology and functional exploration.; Authors - Yanyan Zhang
rat	IF	Front Immunol; Hydrogen sulfide as a therapeutic agent for diabetic wounds: effects on inflammation and fibroblast pyroptosis.; Authors - Fusheng Zhao
mouse	WB,IF	J Nanobiotechnology; Citrate-coated Prussian blue nanozyme hitchhikes neutrophils to ameliorate calcium oxalate crystal-induced kidney injury via inhibiting pyroptosis and NETosis.; Authors - Xiaozhuo Ba