Tested Applications
| Positive WB detected in | mouse spleen tissue, mouse thymus tissue, rat spleen tissue, rat thymus tissue |
| Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30641-1-AP targets ASC/TMS1 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Reactivity | mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32519 Product name: Recombinant mouse PYCARD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-193 aa of NM_023258 Sequence: MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS Predict reactive species |
| Full Name | PYD and CARD domain containing |
| Calculated Molecular Weight | 21KD |
| Observed Molecular Weight | 21-25 kDa |
| GenBank Accession Number | NM_023258 |
| Gene Symbol | Pycard |
| Gene ID (NCBI) | 66824 |
| RRID | AB_3086379 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9EPB4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PYCARD (PYD and CARD domain containing), also known as ASC and CARD5. The calculated molecular weight of PYCARD is 21 kDa. It is located in cytosol, extracellular region and nucleus. PYCARD is also expressed in several structures, including alimentary system; brain; heart; hemolymphoid system gland; and urinary system. The gene enables identical protein binding activity and protein dimerization activity. Involved in several processes, including activation of cysteine-type endopeptidase activity; positive regulation of cytokine production; and regulation of defense response. It acts upstream of or within several processes, including defense response to Gram-positive bacterium; positive regulation of macromolecule metabolic process; and regulation of autophagy. It is also a part of inflammasome complex. Finally, it orthologous to human PYCARD (PYD and CARD domain containing).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for ASC/TMS1 antibody 30641-1-AP | Download protocol |
| IHC protocol for ASC/TMS1 antibody 30641-1-AP | Download protocol |
| WB protocol for ASC/TMS1 antibody 30641-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ASC/TMS1 Polyclonal antibody (Cat# 30641-1-AP) has 39 citations across journals including J Adv Res, J Nanobiotechnology, Adv Sci, Phytomedicine, Int Immunopharmacol, Front Pharmacol, Front Immunol, Brain Res Bull, Cell Signal, and others. Research fields span pyroptosis, NLRP3 inflammasome activation, neuroinflammation, liver and kidney injury, cardiovascular disease, diabetes, osteoarthritis, ulcerative colitis, sepsis, and depression, with applications primarily in WB and IF.
| Species | Application | Title |
|---|---|---|
J Adv Res Engineering a multifunctional nanoplatform with cascade catalytic and bidirectional immunomodulatory capacities for integrating sepsis therapy. | ||
J Nanobiotechnology Citrate-coated Prussian blue nanozyme hitchhikes neutrophils to ameliorate calcium oxalate crystal-induced kidney injury via inhibiting pyroptosis and NETosis. | ||
J Nanobiotechnology Targeting VSMCs to suppress macrophage-like phenotype switching that restrains atherogenesis by immunosuppressive oligodeoxynucleotide (A151) functionalized selenium nanoparticles. | ||
Adv Sci (Weinh) Artesunate Ameliorates APAP-induced Liver Injury by Promoting NEDD4L-Mediated Ubiquitination and Degradation of TXNIP. | ||
Phytomedicine Jiangu formula modulates the gut microbiota structure and the NF-κB/NLRP3 signaling pathway to alleviate synovitis. | ||
Phytomedicine Total paeony glycoside relieves neuroinflammation to exert antidepressant effect via the interplay between NLRP3 inflammasome, pyroptosis and autophagy. |
