Mouse ASC/TMS1 Recombinant monoclonal antibody, PBS Only (Detector)

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  Sandwich ELISA sample test of MP00782-4

  Mouse spleen and mouse thymus tissue lysates were measured. The mouse ASC/TMS1 concentration of detected samples was determined to be 214.92 ng/mL (based on a 2.90 mg/mL extract load) in mouse spleen tissue lysate and 438.45 ng/mL (based on a 6.00 mg/mL extract load) in mouse thymus tissue lysate.

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  Sandwich ELISA standard curve of MP00782-4

  Sandwich ELISA standard curve of MP00782-4, Mouse ASC/TMS1 Recombinant Matched Antibody Pair - PBS only. 83858-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32519. 83858-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL

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  Affinity and Kinetic Characterization of 83858-1-PBS

  Biolayer interferometry (BLl) kinetic assays of 83858-1-PBS against Mouse ASC/TMS1 were performed. The affinity constant is 2.27 nM.

ASC/TMS1 Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 83858-1-PBS

Clone No.240908B7

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

Pycard, Apoptosis-associated speck-like protein containing a CARD, Asc, CARD5, masc

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Product Information

83858-1-PBS targets ASC/TMS1 as part of a matched antibody pair:

MP00782-4: 83858-2-PBS capture and 83858-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag32519

  Product name: Recombinant mouse PYCARD protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-193 aa of NM_023258

  Sequence: MGRARDAILDALENLSGDELKKFKMKLLTVQLREGYGRIPRGALLQMDAIDLTDKLVSYYLESYGLELTMTVLRDMGLQELAEQLQTTKEESGAVAAAASVPAQSTARTGHFVDQHRQALIARVTEVDGVLDALHGSVLTEGQYQAVRAETTSQDKMRKLFSFVPSWNLTCKDSLLQALKEIHPYLVMDLEQS

• Full Name: PYD and CARD domain containing
• Calculated Molecular Weight: 21KD
• GenBank Accession Number: NM_023258
• Gene Symbol: Pycard
• Gene ID (NCBI): 66824
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9EPB4
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.