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  • KD/KO Validated

PYCR2 Polyclonal antibody

PYCR2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 17146-1-AP


P5C reductase 2, P5CR 2, P5CR2, PYCR2

Tested Applications

Positive WB detected inHeLa cells, L02 cells, Jurkat cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human placenta tissue, human kidney tissue, human testis tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:1500-1:6000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17146-1-AP targets PYCR2 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity African green monkey, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PYCR2 fusion protein Ag10918
Full Name pyrroline-5-carboxylate reductase family, member 2
Calculated molecular weight 320 aa, 34 kDa
Observed molecular weight 34 kDa
GenBank accession numberBC020553
Gene symbol PYCR2
Gene ID (NCBI) 29920
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PYCR2, also named as P5CR2, belongs to the pyrroline-5-carboxylate reductase family. It catalyses the reaction: L-proline + NAD(P)+ = 1-pyrroline-5-carboxylate + NAD(P)H. This antibody may have cross reaction to PYCR1 due to the high homology.


Product Specific Protocols
WB protocol for PYCR2 antibody 17146-1-APDownload protocol
IHC protocol for PYCR2 antibody 17146-1-APDownload protocol
IF protocol for PYCR2 antibody 17146-1-APDownload protocol
IP protocol for PYCR2 antibody 17146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Aging Dis

A Transcriptome Study of Progeroid Neurocutaneous Syndrome Reveals POSTN As a New Element in Proline Metabolic Disorder.

Authors - Yu-Wen Huang

Nat Commun

Kindlin-2 links mechano-environment to proline synthesis and tumor growth.

Authors - Ling Guo

Am J Respir Cell Mol Biol

Glutamine Metabolism is Required for Collagen Protein Synthesis in Lung Fibroblasts.

Authors - Robert B Hamanaka
African green monkeyWB

Virol Sin

African Swine Fever Virus Protein E199L Promotes Cell Autophagy through the Interaction of PYCR2.

Authors - Sheng Chen
  • KD Validated

Nat Metab

Mitochondrial NADP+ is essential for proline biosynthesis during cell growth.

Authors - Diem H Tran

Sci Rep

Activation of proline biosynthesis is critical to maintain glutamate homeostasis during acute methamphetamine exposure.

Authors - Bobby Jones


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Iram (Verified Customer) (07-03-2020)

Giving a very sharp and clean result

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Human