PYCRL Polyclonal antibody, PBS Only

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• PYCRL Polyclonal antibody, PBS Only (16008-1-PBS)

• 

  WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16008-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16008-1-AP (same clone as 16008-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16008-1-AP (PYCRL antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16008-1-PBS in a different storage buffer formulation.

PYCRL Polyclonal Antibody for WB, Indirect ELISA

Cat No. 16008-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

PYCR3, EC:1.5.1.2, P5C reductase 3, P5CR 3, Pyrroline-5-carboxylate reductase 3

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Product Information

16008-1-PBS targets PYCRL in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8716

  Product name: Recombinant human PYCRL protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-274 aa of BC007993

  Sequence: MAAAEPSPRRVGFVGAGRMAGAIAQGLIRAGKVEAQHILASAPTDRNLCHFQALGCRTTHSNQEVLQSCLLVIFATKPHVLPAVLAEVAPVVTTEHILVSVAAGVSLSTLEELLPPNTRVLRVLPNLPCVVQEGAIVMARGRHVGSSETNLLQHLLEACGRCEEVPEAYVDIHTGLSGSGVAFVCAFSEALAEGAVKMGMPSSLAHRIAAQTLLGTAKMLLHEGQHPAQLRSDVCTPGGTTIYGLHALEQGGLRAATMSAVEAATCRAKELSRK

• Full Name: pyrroline-5-carboxylate reductase-like
• Calculated Molecular Weight: 274 aa, 29 kDa
• Observed Molecular Weight: 29 kDa
• GenBank Accession Number: BC007993
• Gene Symbol: PYCRL
• Gene ID (NCBI): 65263
• RRID: AB_3669228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q53H96
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Proline conversion into P5C is coupled to the P5C reduction into proline, catalyzed by P5C reductase. This enzyme has three isoforms: PYCR1/PYCR2, which are specific for the mitochondrial compartment and the PYCRL present in cytosol. The conversion of P5C to proline, and its transport between mitochondria and cytosol, are also coupled with glucose metabolism by the pentose phosphate pathway.(PMID: 35163433)