PYGL Polyclonal antibody

PYGL Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 55429-1-AP

Synonyms

GSD6, phosphorylase, glycogen, liver, PYGL

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Validation Data Gallery

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  WB analysis of HepG2 using 55429-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 55429-1-AP (PYGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 55429-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 55429-1-AP (PYGL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 55429-1-AP

  IP result of anti-PYGL (IP:55429-1-AP, 4ug; Detection:55429-1-AP 1:500) with HepG2 cells lysate 2400ug.

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  IHC staining of human skeletal muscle using 55429-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55429-1-AP (PYGL antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 55429-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 55429-1-AP (PYGL antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 55429-1-AP

  Immunofluorescent analysis of HepG2 cells using 55429-1-AP (PYGL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells, mouse liver
• Positive IP detected in: HepG2 cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55429-1-AP targets PYGL in WB, IP, IF,ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: phosphorylase, glycogen, liver
• Calculated Molecular Weight: 97 kDa
• Observed Molecular Weight: 97-105 kDa
• GenBank Accession Number: NM_002863
• Gene Symbol: PYGL
• Gene ID (NCBI): 5836
• RRID: AB_11182599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PYGL belongs to the glycogen phosphorylase family. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties. Defects in PYGL are the cause of glycogen storage disease type 6 (GSD6). This antibody is specific to PYGL. It has no cross reaction to PYGM and PYGB.