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PYGO2 Polyclonal antibody, PBS Only (11555-1-PBS)

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WB analysis of MDA-MB-453s using 11555-1-AP (same clone as 11555-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11555-1-AP (same clone as 11555-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.
WB analysis of C6 using 11555-1-AP (same clone as 11555-1-PBS)
C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.
WB analysis of C6 using 11555-1-AP (same clone as 11555-1-PBS)
C6 cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11555-1-AP (same clone as 11555-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.
WB analysis of MDA-MB-453s using 11555-1-AP (same clone as 11555-1-PBS)
MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11555-1-AP (PYGO2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 11555-1-AP (same clone as 11555-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11555-1-AP (PYGO2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11555-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

PYGO2 Polyclonal antibody, PBS Only

Name: PYGO2 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11555-1-PBS

PYGO2 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 11555-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

PYGO 2, PP7910

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11555-1-PBS targets PYGO2 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2111 Product name: Recombinant human PYGO2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 101-406 aa of BC032099 Sequence: FGGFRVQGGMAGQVPPGYSTGGGGGPQPLRRQPPPFPPNPMGPAFNMPPQGPGYPPPGNMNFPSQPFNQPLGQNFSPPSGQMMPGPVGGFGPMISPTMGQPPRAELGPPSLSQRFAQPGAPFGPSPLQRPGQGLPSLPPNTSPFPGPDPGFPGPGGEDGGKPLNPPASTAFPQEPHSGSPAAAVNGNQPSFPPNSSGRGGGTPDANSLAPPGKAGGGSGPQPPPGLVYPCGACRSEVNDDQDAILCEASCQKWFHRECTGMTESAYGLLTTEASAVWACDLCLKTKEIQSVYIREGMGQLVAANDG Predict reactive species
Full Name	pygopus homolog 2 (Drosophila)
Calculated Molecular Weight	406 aa, 41 kDa
Observed Molecular Weight	50 kDa
GenBank Accession Number	BC032099
Gene Symbol	PYGO2
Gene ID (NCBI)	90780
RRID	AB_2877776
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9BRQ0
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PYGO2, also named as Pygopus homolog 2, is a 406 animo acid protein, which contains 1 PHD-type zinc finger. PYGO2 is involved in signal transduction through the Wnt pathway and Binds to BCL9 via the PHD-type zinc finger motif, and thereby becomes part of the nuclear beta-catenin/TCF complex. PYGO2 is in a high proportion of breast and epithelial ovarian malignant tumors and is required for the growth of several cell lines derived from these carcinomas.