PE-conjugated Pan-Keratin (TypeⅡ) Recombinant monoclonal antibody

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• PE-conjugated Pan-Keratin (TypeⅡ) Recombinant monoclonal antibody (PE-86370)

Pan-Keratin (TypeⅡ) Uni-rAb^® Recombinant Antibody for IF-P

Cat No. PE-86370

Clone No.251040F7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Synonyms

Pan Keratin, Pan-Keratin, Type II (basic/neutral), Type II cytokeratin, Type II cytokeratins

• 

  IF Staining of human colon cancer using PE-86370

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human colon cancer using PE-86370

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human cervical cancer using PE-86370

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human cervical cancer using PE-86370

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human cervical cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human lung cancer using PE-86370

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of human lung cancer using PE-86370

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using PE Pan-Keratin (TypeⅡ) antibody (PE-86370, Clone: 251040F7 ) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IF-P detected in: human colon cancer tissue, human lung cancer tissue, human cervical cancer tissue

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

PE-86370 targets Pan-Keratin (TypeⅡ) in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: Pan-Keratin (TypeⅡ)
• Observed Molecular Weight: 52-68 kDa
• Conjugate: PE Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 496 nm, 565 nm / 578 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q14533
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Keratin is a family of fibrous structural proteins that are key components in hair, nails, feathers, hooves, and the outer layer of skin (epidermis). There are two main types of keratin: Type I (acidic) and Type II (basic/neutral), which form heterodimers to create intermediate filaments, providing mechanical strength to cells. Type II keratins (or Type II cytokeratins) are basic or neutral proteins that form part of the intermediate filament cytoskeleton in epithelial cells. Type II keratins include K1-K8, and K71-K86. 86370-1-RR can recognize KRT81/82/83/85/86.