Phospho-AMPK Alpha (Thr172) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 80209-6-RR (same clone as 80209-6-PBS)

  Non-treated C2C12 cells and H2O2 treated C2C12 cells were subjected to SDS PAGE followed by western blot with 80209-6-RR (Phospho-AMPK Alpha (Thr172) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80209-6-PBS in a different storage buffer formulation.

• 

  WB analysis using 80209-6-RR (same clone as 80209-6-PBS)

  Non-treated, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80209-6-RR (Phospho-AMPK Alpha (Thr172) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80209-6-PBS in a different storage buffer formulation.

Phospho-AMPK Alpha (Thr172) Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 80209-6-PBS

Clone No.242923D4

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

P-AMPK Alpha 2 (Thr172), Phos AMPK Alpha, Phos-AMPK Alpha 1 (Thr183), Phos-AMPK Alpha 2 (Thr172), Phospho-AMPK Alpha 1 (Thr183)

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Product Information

80209-6-PBS targets Phospho-AMPK Alpha (Thr172) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC048980
• Gene Symbol: AMPK Alpha 1
• Gene ID (NCBI): 5562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13131
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

AMPK is a serine/threonine protein kinase complex consisting of a catalytic α-subunit (α1 and α2), a scaffolding β-subunit (β1 and β2), and a regulatory γ-subunit (γ1, γ2, and γ3). Ubiquitous expression of AMPKα1-, β1-, and γ1-subunits in many tissues makes the α1β1γ1 complex a reference for AMPK assays to identify AMPK activators. AMPK is generally quiescent under normal conditions but is activated in response to signals and stresses that increase the AMP/ATP ratio, such as hypoglycemia, strenuous exercise, anoxia, and ischemia. An increase in the ratio of AMP/ATP activates AMPK by several mechanisms, including direct allosteric activation and covalent modification in which an AMP-dependent AMPK kinase (AMPKK) phosphorylates the α subunit on Thr172. Once activated, AMPK switches on catabolic pathways that generate ATP, while switching off ATP-consuming processes (e.g., biosynthesis, cell growth, and proliferation), and in doing so acts as an "energy gauge". (PMID: 27034026, PMID: 21980456, PMID: 27600021)
This antibody can recognize the phosphorylation sites of Thr183 in AMPK Alpha 1 and Thr172 in AMPK Alpha 2.