Phospho-AMPK Alpha (Thr172) Recombinant monoclonal antibody

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  WB analysis using 80209-6-RR

  Non-treated C2C12 cells and H2O2 treated C2C12 cells were subjected to SDS PAGE followed by western blot with 80209-6-RR (Phospho-AMPK Alpha (Thr172) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

• 

  WB analysis using 80209-6-RR

  Non-treated, phosphatase inhibitor treated and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 80209-6-RR (Phospho-AMPK Alpha (Thr172) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control.

Phospho-AMPK Alpha (Thr172) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 80209-6-RR

Clone No.242923D4

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

P-AMPK Alpha 2 (Thr172), Phos AMPK Alpha, Phos-AMPK Alpha 1 (Thr183), Phos-AMPK Alpha 2 (Thr172), Phospho-AMPK Alpha 1 (Thr183)

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Tested Applications

• Positive WB detected in: H2O2 treated C2C12 cells, λ phosphatase treated HEK-293 cells

This antibody can recognize the phosphorylation sites of Thr183 in AMPK Alpha 1 and Thr172 in AMPK Alpha 2.

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

80209-6-RR targets Phospho-AMPK Alpha (Thr172) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: protein kinase, AMP-activated, alpha 1 catalytic subunit
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC048980
• Gene Symbol: AMPK Alpha 1
• Gene ID (NCBI): 5562
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13131
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AMPK is a serine/threonine protein kinase complex consisting of a catalytic α-subunit (α1 and α2), a scaffolding β-subunit (β1 and β2), and a regulatory γ-subunit (γ1, γ2, and γ3). Ubiquitous expression of AMPKα1-, β1-, and γ1-subunits in many tissues makes the α1β1γ1 complex a reference for AMPK assays to identify AMPK activators. AMPK is generally quiescent under normal conditions but is activated in response to signals and stresses that increase the AMP/ATP ratio, such as hypoglycemia, strenuous exercise, anoxia, and ischemia. An increase in the ratio of AMP/ATP activates AMPK by several mechanisms, including direct allosteric activation and covalent modification in which an AMP-dependent AMPK kinase (AMPKK) phosphorylates the α subunit on Thr172. Once activated, AMPK switches on catabolic pathways that generate ATP, while switching off ATP-consuming processes (e.g., biosynthesis, cell growth, and proliferation), and in doing so acts as an "energy gauge". (PMID: 27034026, PMID: 21980456, PMID: 27600021)
This antibody can recognize the phosphorylation sites of Thr183 in AMPK Alpha 1 and Thr172 in AMPK Alpha 2.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Med; Celastrol improves endothelial function in diet-induced obesity mice via attenuating endoplasmic reticulum stress through the activation of AMPK pathway; Authors - Cheng Yu
human	WB	BMC Cardiovasc Disord; Mechanism of adenosine monophosphate-activated protein kinase promoting lower extremity varicose veins development via cytoskeletal dynamics; Authors - Yongmei Zhang