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CoraLite® Plus 647-conjugated Phospho-BAD (Ser112) Recombinant monoclonal antibody (CL647-80431-2)

CoraLite® Plus 647-conjugated Phospho-BAD (Ser112) Recombinant monoclonal antibody

Name: CoraLite® Plus 647-conjugated Phospho-BAD (Ser112) Recombinant monoclonal antibody, 100ul
Brand: Proteintech
SKU: CL647-80431-2-100UL

Phospho-BAD (Ser112) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-80431-2

Clone No. 251074F11

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

BAD (Ser112), p BAD (Ser112), p-BAD, p-BAD (Ser112), p-BAD(Ser112)

Formulation: PBS, Proclin300, BSA, Glycerol

PBS, Proclin300, BSA, Glycerol

Conjugate: CoraLite® Plus 647

Unconjugated CoraLite® Plus 488 CoraLite® Plus 647

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Filter: AllFC (Intra)

FC experiment of HEK-293 using CL647-80431-2
1X10^6 HEK-293 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-BAD (Ser112) Recombinant Antibody (CL647-80431-2, Clone:251074F11)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
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MSDS SDS

Tested Applications

Positive FC (Intra) detected in

HEK-293 cells

Recommended dilution

Application	Dilution
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL647-80431-2 targets Phospho-BAD (Ser112) in FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	BCL2-associated agonist of cell death
Calculated Molecular Weight	18 kDa
Observed Molecular Weight	25 kDa
GenBank Accession Number	BC001901
Gene Symbol	BAD
Gene ID (NCBI)	572
Conjugate	CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths	654 nm / 674 nm
Excitation Laser	Red Laser (633 nm)
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q92934
Storage Buffer	PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage Conditions	Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BAD, a distant member of the BCL-2 family, exerts its death promoting e€ect by hetero-dimerizing with BCL-XL and BCL-2 death antagonists in the mitochondria. The function of the pro-apoptotic molecule BAD is regulated by phosphorylation of two sites, serine-112 (Ser-112) and serine-136 (Ser-136). Phosphorylation at either site results in loss of the ability of BAD to heterodimerize with the survival proteins BCL-XL or BCL-2. Phosphorylated BAD binds to 14-3-3 and is sequestered in the cytoplasm. (PMID: 10597268)