Phospho-BCR (Tyr177) Recombinant monoclonal antibody

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  WB analysis using 87327-1-RR

  K-562 cell lysates were subjected to SDS PAGE followed by western blot with 87327-1-RR (Phospho-BCR (Tyr177) antibody) blocked with BSA only, Phospho-BCR (Tyr177) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Lamin B1 (66095-1-Ig) antibody as a loading control.

Phospho-BCR (Tyr177) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87327-1-RR

Clone No.252641D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

BCR (Tyr177), p-BCR (Tyr177), p-BCR (Y177), p-BCR Tyr177, Phospho BCR (Tyr177)

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87327-1-RR targets Phospho-BCR (Tyr177) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: breakpoint cluster region
• Calculated Molecular Weight: 143 kDa
• Observed Molecular Weight: 210 kDa
• GenBank Accession Number: NM_004327
• Gene Symbol: BCR
• Gene ID (NCBI): 613
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P11274
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The Bcr gene, mapping on chromosome22, was orginally identified by its presence in the chimeric Bcr-Abl oncogene. The aminoterminal BCR-encoded sequences of BCR-ABL contain a tyrosine-phosphorylated site that binds the SH2 domain of the adaptor protein GRB2. It is now evident that the phosphorylation of BCR Tyr177 is essential for BCR-ABL-mediated leukemogenesis, and its mutation largely abolishes GRB2 binding and diminishes BCR-ABL-induced Ras activation. (PMID: 22156549)