Phospho-Beta Catenin (Ser675) Polyclonal antibody

Phospho-Beta Catenin (Ser675) Polyclonal Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (3)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 28853-1-AP

Synonyms

b cat, Beta catenin, Catenin beta 1, CTNNB, CTNNB1, DKFZp686D02253, FLJ25606, FLJ37923, Phospho-Beta Catenin (Ser675), PRO2286, β-Catenin



Tested Applications

Positive WB detected inλ phosphatase treated HT-29 cells
Positive IF detected inλ phosphatase treated MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 8 publications below

Product Information

28853-1-AP targets Phospho-Beta Catenin (Ser675) in WB, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name catenin (cadherin-associated protein), beta 1, 88kDa
Calculated Molecular Weight 781 aa, 86 kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberBC058926
Gene Symbol CTNNB1
Gene ID (NCBI) 1499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. Beta catenin is a core component of the cadherin protein complex, whose stabilization is essential for the activation of Wnt/β-catenin signaling. Phosphorylation of Ser675 in the C-terminus of at beta catenin promotesmits nuclear accumulation and transcriptional activity, resulting in increased activation of the Wnt/β-catenin signaling pathway(PMID: 25980439). In addition, Ser675 phosphorylation promotes beta catenin interaction with the transcriptional coactivator CREB-binding protein in the nucleus(PMID: 16476742).

Protocols

Product Specific Protocols
WB protocol for Phospho-Beta Catenin (Ser675) antibody 28853-1-APDownload protocol
IF protocol for Phospho-Beta Catenin (Ser675) antibody 28853-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Endocrinology

Casein kinase 1α regulates testosterone synthesis and testis development in adult mice

Authors - Hongzhou Guo
humanWB

FASEB J

Verteporfin attenuates trauma-induced heterotopic ossification of Achilles tendon by inhibiting osteogenesis and angiogenesis involving YAP/β-catenin signaling

Authors - Gang Luo
humanWB

Bone

Osteoblast-derived exosomes promote osteogenic differentiation of osteosarcoma cells via URG4/Wnt signaling pathway

Authors - Yuanxi Leng
bovineWB

Cells

DPPA2/4 Promote the Pluripotency and Proliferation of Bovine Extended Pluripotent Stem Cells by Upregulating the PI3K/AKT/GSK3β/β-Catenin Signaling Pathway

Authors - Shu Fang
humanWB

Exp Mol Med

Advanced glycation end products promote meniscal calcification by activating the mTOR-ATF4 positive feedback loop

Authors - Sheng Yang
humanWB

Cell Rep

LncRNA DGCR5-encoded polypeptide RIP aggravates SONFH by repressing nuclear localization of β-catenin in BMSCs

Authors - Weiqian Jiang