Phospho-CAD (Ser1859) Recombinant monoclonal antibody

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• Phospho-CAD (Ser1859) Recombinant monoclonal antibody (87337-1-RR)

• 

  WB analysis using 87337-1-RR

  Non-treated HEK-293 cells, phosphatase inhibitor treated HEK-293 cells and λ phosphatase treated HEK-293 cells were subjected to SDS PAGE followed by western blot with 87337-1-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

• 

  WB analysis using 87337-1-RR

  Non-treated HeLa cells, phosphatase inhibitor treated HeLa cells and λ phosphatase treated HeLa cells were subjected to SDS PAGE followed by western blot with 87337-1-RR (Phospho-CAD (Ser1859) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Vinculin (86930-1-RR) antibody as a loading control.

Phospho-CAD (Ser1859) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87337-1-RR

Clone No.252640E4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CAD (Ser1859), p-CAD (S1859), p-CAD (Ser1859), p-CAD Ser1859, Phospho CAD (Ser1859)

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, λ phosphatase treated HEK-293 cells, λ phosphatase treated HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87337-1-RR targets Phospho-CAD (Ser1859) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase
• Calculated Molecular Weight: 2225 aa, 243 kDa
• Observed Molecular Weight: 250 kDa
• GenBank Accession Number: NM_004341
• Gene Symbol: CAD
• Gene ID (NCBI): 790
• RRID: AB_3745476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P27708
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamoylase, and dihydroorotase) is a multifunctional enzyme required for the de novo synthesis of pyrimidine nucleotides. Phospho-CAD (Ser1859) is a phosphorylated form of the CAD (carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase) enzyme at Serine 1859. This site is specifically phosphorylated by mTOR complex 1 (mTORC1), acting through the downstream kinase S6K1, and plays a role in regulating pyrimidine biosynthesis. The phosphorylation of CAD at Ser1859 is a part of the PI3K-AKT-mTORC1-S6K1 pathway that integrates growth signals to regulate cell growth, proliferation, and homeostasis by modulating several anabolic and catabolic processes.In addition, insulin also stimulates the Ser1859 phosphorylation of CAD in a manner sensitive to rapamycin as well as phosphatase and tensin homolog (PTEN) deletion in the PI3K-AKT pathway. (PMID:25422319, PMID: 34638594)