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Phospho-CBX3 (Ser93) Recombinant monoclonal antibody, PBS Only (87869-3-PBS)

Phospho-CBX3 (Ser93) Recombinant monoclonal antibody, PBS Only

Name: Phospho-CBX3 (Ser93) Recombinant monoclonal antibody, PBS Only, 100ug
Brand: Proteintech
SKU: 87869-3-PBS-100UG

Phospho-CBX3 (Ser93) Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Indirect ELISA

Cat No. 87869-3-PBS

Clone No.260091A2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Indirect ELISA

Synonyms

CBX3 (Ser93), HP1 gamma (Ser83), p-CBX3, p-CBX3 (Ser93), Phospho-CBX3

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

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Filter: AllWBFC (Intra)

WB analysis using 87869-3-RR (same clone as 87869-3-PBS)
Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87869-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated HeLa cells and paclitaxel treated HeLa cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87869-3-PBS in a different storage buffer formulation.
WB analysis using 87869-3-RR (same clone as 87869-3-PBS)
Non-treated NIH/3T3 cells and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87869-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
Non-treated NIH/3T3 cells and Forskolin treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 87869-3-RR (Phospho-CBX3 (Ser93) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 87869-3-PBS in a different storage buffer formulation.
FC experiment of HeLa using 87869-3-RR (same clone as 87869-3-PBS)
1x10^6 HeLa cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-CBX3 (Ser93) Recombinant monoclonal antibody (87869-3-RR, Clone:260091A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87869-3-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
×
1x10^6 HeLa cells untreated (dashed lines) or treated with paclitaxel which intracellularly stained with 0.13 ug Phospho-CBX3 (Ser93) Recombinant monoclonal antibody (87869-3-RR, Clone:260091A2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 87869-3-PBS in a different storage buffer formulation.

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

87869-3-PBS targets Phospho-CBX3 (Ser93) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	chromobox homolog 3 (HP1 gamma homolog, Drosophila)
Calculated Molecular Weight	21 kDa
Observed Molecular Weight	22 kDa
GenBank Accession Number	NM_016587
Gene Symbol	CBX3
Gene ID (NCBI)	11335
RRID	AB_3745797
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q13185
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

CBX3, also known as HP1γ (Heterochromatin Protein 1 gamma), is a protein-coding gene located on human chromosome 7p15.2. Phospho-CBX3 (Ser93) refers to the phosphorylated form of Chromobox Homolog 3 (CBX3), also known as HP1γ (Heterochromatin Protein 1 gamma), at serine residue 93. This phosphorylation site is located in the N-terminal tail region of the protein and plays a regulatory role in chromatin binding and transcriptional control. (PMID: 39907700)