Phospho-CrkL (Tyr207) Recombinant antibody

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  WB analysis of NIH/3T3 using 84590-2-RR

  Non-treated NIH/3T3 cells and Blocked Phosphorylated peptide treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 84590-2-RR (Phospho-CrkL (Tyr207) antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

Phospho-CrkL (Tyr207) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 84590-2-RR

Clone No.240881A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CrkL (Tyr207), p CrkL, p CrkL (Tyr207), p-CrkL, p-CrkL (Tyr207)

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Tested Applications

• Positive WB detected in: NIH/3T3 cells, Blocked Phosphorylated peptide treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84590-2-RR targets Phospho-CrkL (Tyr207) in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: v-crk sarcoma virus CT10 oncogene homolog (avian)-like
• Calculated Molecular Weight: 34 kDa
• GenBank Accession Number: BC043500
• Gene Symbol: CRKL
• Gene ID (NCBI): 1399
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P46109
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CrkL (v-crk avian sarcoma virus CT10 oncogene homolog-like) is a cytoplasmic adaptor protein that belongs to the Crk family of adaptors. Similar to other adaptor proteins, Crk functions primarily to assemble molecular complexes to distribute signals within the cell. The best studied regulatory mechanism of Crk proteins is the phosphorylation at a tyrosine located in a linker between the N- and C-terminal SH3 domains (Tyr221 in human CrkII, Tyr207 in human CrkL). (PMID: 24675776)