Phospho-Cyclin D1 (Thr286) Recombinant monoclonal antibody

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  WB analysis using 85246-1-RR

  Non-treated NIH/3T3 cells and MG132 treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 85246-1-RR (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

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  WB analysis of HT-1080 using 85246-1-RR

  Non-treated HT-1080 cells and MG132 treated HT-1080 cells were subjected to SDS PAGE followed by western blot with 85246-1-RR (Phospho-Cyclin D1 (Thr286) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-Cyclin D1 (Thr286) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 85246-1-RR

Clone No.242211A1

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, ELISA

Synonyms

Cyclin D1 (Thr286), p Cyclin D1, p Cyclin D1 (Thr286), p-Cyclin D1, p-Cyclin D1 (Thr286)

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Tested Applications

• Positive WB detected in: MG132 treated HT-1080 cells, MG132 treated NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85246-1-RR targets Phospho-Cyclin D1 (Thr286) in WB, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: cyclin D1
• Calculated Molecular Weight: 295 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000076
• Gene Symbol: Cyclin D1
• Gene ID (NCBI): 595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P24385
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Cyclin D1 is an important regulator of G1-S phase cell cycle transition. Active cyclin D1-cyclin dependent kinase 4/6 complexes phosphorylate retinoblastoma protein, resulting in release of sequestered E2F transcription factors and subsequent expression of genes required for progression into S phase. Cyclin D1 was localized to the nucleus and its rapid ubiquitin-dependent degradation shown to require phosphorylation at Thr286 by glycogen synthase kinase 3β (GSK3β). (PMID: 16504004)