CoraLite® Plus 647-conjugated Phospho-EGFR (Tyr1016) Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• CoraLite® Plus 647-conjugated Phospho-EGFR (Tyr1016) Recombinant monoclonal antibody (CL647-82736-11)

Phospho-EGFR (Tyr1016) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-82736-11 Copied!

Clone No. 250687B2

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

EGF Receptor (Tyr992), EGFR (Tyr1016), EGFR (Tyr992), p EGFR (Tyr1016), p-EGF Receptor (Tyr992)

• 

  FC experiment of A431 using CL647-82736-11

  1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-EGFR (Tyr1016) Recombinant Antibody (CL647-82736-11, Clone:250687B2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for Phospho-EGFR (Tyr1016) in ?

Chat with Able

---

Tested Applications

• Positive FC (Intra) detected in: EGF treated A431 cells

This antibody, also known as EGFR Tyr992, is the site where the signal peptide is removed.

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-82736-11 targets Phospho-EGFR (Tyr1016) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
• Calculated Molecular Weight: 1210 aa, 134 kDa
• Observed Molecular Weight: 170 kDa
• GenBank Accession Number: BC094761
• Gene Symbol: EGFR
• Gene ID (NCBI): 1956
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P00533
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Phosphorylation at Tyr1016 is essential for the recruitment of downstream signaling molecules. For example, it can interact with adapter proteins like GRB2, which in turn activates the Ras-MAPK pathway, leading to cell proliferation and differentiation. (PMID: 26628682) Some scientists call EGFR Tyr1016 as EGFR Tyr992, the site where the signaling peptide is removed.