CoraLite® Plus 647-conjugated Phospho-EIF4E (Ser209) Recombinant monoclonal antibody

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• CoraLite® Plus 647-conjugated Phospho-EIF4E (Ser209) Recombinant monoclonal antibody (CL647-84981)

Phospho-EIF4E (Ser209) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL647-84981 Copied!

Clone No. 242049G10

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

EIF4E (Ser209), p-EIF4E, p-EIF4E (Ser209), Phospho-EIF4E, Phospho-EIF4E Ser209

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  FC experiment of HEK-293 using CL647-84981

  1X10^6 HEK-293 cells untreated (dashed lines) or treated with Calyculin A which intracellularly stained with 0.25 ug Coralite® Plus 647 Phospho-Eif4e (Ser209) Recombinant Antibody (CL647-84981, Clone:242049G10)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: Calyculin A treated HEK-293 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL647-84981 targets Phospho-EIF4E (Ser209) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: eukaryotic translation initiation factor 4E
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: BC012611
• Gene Symbol: EIF4E
• Gene ID (NCBI): 1977
• Conjugate: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 654 nm / 674 nm
• Excitation Laser: Red Laser (633 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P06730
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Eukaryotic initiation factor 4F (eIF4F) is a critical effector and is thought to be the rate-limiting determinant of translation. The activity of eIF4E can be regulated by phosphorylation at Ser209, which is controlled by mitogen-activated protein kinase-interacting kinases Mnk1 and Mnk2. Although the function of eIF4E phosphorylation remains the subject of debate, it has been documented that the phosphorylation of eIF4E at Ser209 is crucial for biogenesis, tumorigenesis, and viral infection. (PMID: 38299843)