CoraLite® Plus 488-conjugated Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody

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• CoraLite® Plus 488-conjugated Phospho-Estrogen Receptor α (Ser118) Recombinant monoclonal antibody (CL488-84564-6)

Phospho-Estrogen Receptor α (Ser118) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-84564-6 Copied!

Clone No. 250278F8

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra)

Synonyms

ESR1, ER, ER alpha, Era, ER-alpha

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  FC experiment of MCF-7 using CL488-84564-6

  1X10^6 MCF-7 cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.13 ug Coralite® Plus 488 Phospho-Estrogen Receptor Α (Ser118) Recombinant Antibody (CL488-84564-6, Clone:250278F8)(red), or 0.13 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC (Intra) detected in: MCF-7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84564-6 targets Phospho-Estrogen Receptor α (Ser118) in FC (Intra) applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: estrogen receptor 1
• Calculated Molecular Weight: 595 aa, 66 kDa
• Observed Molecular Weight: 66 kDa
• GenBank Accession Number: BC128573
• Gene Symbol: ER
• Gene ID (NCBI): 2099
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P03372
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Estrogen Receptor α (ERα, gene symbol ESR1) is a member of the nuclear-receptor superfamily that functions as a ligand-dependent transcription factor. Dependent upon cell and promoter context, ERα regulates transcription through its two activation domains, AF-1 and AF-2. AF-2 is contained within the LBD and becomes active upon E2 binding. AF-1 however, is activated by phosphorylation of ERα at several sites, most notably serine 118 (S118) and serine 167 (S167), sites that are regulated by multiple signaling pathways. (PMID: 25597633)