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Phospho-FOXO1 (Ser319) Recombinant monoclonal antibody (82997-7-RR)

Phospho-FOXO1 (Ser319) Recombinant monoclonal antibody

Phospho-FOXO1 (Ser319) Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 82997-7-RR

Clone No.250275C3

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FOXO1 (Ser319), p FOXO1 (Ser319), p-FOXO1, p-FOXO1 (Ser319), p-FOXO1(Ser319)

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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WB analysis using 82997-7-RR
HEK-293T and MCF-7 cell lysates were subjected to SDS PAGE followed by western blot with 82997-7-RR (Phospho-FOXO1 (Ser319) antibody) blocked with BSA only, Phospho-FOXO1 (Ser319) peptide or non-Phospho peptide at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in

MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82997-7-RR targets Phospho-FOXO1 (Ser319) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	forkhead box O1
Calculated Molecular Weight	655 aa, 70 kDa
Observed Molecular Weight	70-80 kDa
GenBank Accession Number	BC021981
Gene Symbol	FOXO1
Gene ID (NCBI)	2308
RRID	AB_3743451
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q12778
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The forkhead box class O (FoxO) family in mammals is comprised of the evolutionally highly conserved forkhead transcription factors, FoxO1, FoxO3a, FoxO4, and FoxO6. FoxO1 is highly expressed in lymphoid cells, especially in T and B cells. FoxO1 phosphorylation determines the transcriptional activity and cellular localization of FoxO1. Results showed that GLK-IKKβ signaling triggered the nuclear export of FoxO1. (PMID: 35966593)