CoraLite® Plus 488-conjugated Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody

• Home
• Products
• Primary Antibodies
• CoraLite® Plus 488-conjugated Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Recombinant monoclonal antibody (CL488-82371-5)

Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) Uni-rAb^® Recombinant Antibody for FC (Intra)

Cat No. CL488-82371-5 Copied!

Clone No. 242663E11

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

FC (Intra)

Synonyms

P-GSK3A (Tyr279), P-GSK3B (Tyr216), Phos-GSK3 beta (Tyr216), Phos-GSK3A (Tyr279), Phos-GSK3B (Tyr216)

• 

  FC experiment of HEK-293T using CL488-82371-5

  1X10^6 HEK-293T cells untreated (dashed lines) or treated with λ phosphatase which intracellularly stained with 0.25 ug Coralite® Plus 488 Phospho-Gsk3a (Tyr279)/Gsk3b (Tyr216) Recombinant Antibody (CL488-82371-5, Clone:242663E11)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) in ?

Chat with Able

---

Tested Applications

• Positive FC (Intra) detected in: λ phosphatase treated HEK-293T cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-82371-5 targets Phospho-GSK3A (Tyr279)/GSK3B (Tyr216) in FC (Intra) applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: glycogen synthase kinase 3 beta
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC000251
• Gene Symbol: GSK3B
• Gene ID (NCBI): 2932
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P49841
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Glycogen synthase kinase 3 (GSK3) proteins are serine/threonine kinases that were originally identified as key regulatory enzymes in glucose metabolism. There are two isoforms, GSK3 alpha and GSK3 beta, that are encoded by separate genes and that are 85% homologous to each other overall with 95% homology in the kinase domains. GSK3 beta is activated by phosphorylation of the tyrosine 216 residue (Tyr216) located in the kinase domain and inactivated by phosphorylation of the amino-terminal serine 9 residue (Ser9). GSK3 phosphorylation inhibits its catalytic activity towards its substrates, allowing cytosolic stabilization and nuclear translocation of the transcriptional targets, β-catenin and Nrf2. (PMID: 31354196 , PMID: 25750704,PMID: 24722055)