Phospho-HER2/ErbB2 (Tyr877) Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82762-11-RR (same clone as 82762-11-PBS)

  Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 82762-11-RR (Phospho-HER2/ErbB2 (Tyr877) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 82762-11-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 82762-11-RR (same clone as 82762-11-PBS)

  Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-HER2/ErbB2 (Tyr877) antibody (82762-11-RR, Clone: 250539F5 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82762-11-PBS in a different storage buffer formulation.

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  FC experiment of A431 using 82762-11-RR (same clone as 82762-11-PBS)

  1X10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-HER2/ErbB2 (Tyr877) Recombinant monoclonal antibody (82762-11-RR, Clone:250539F5) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Flow Cytometry Phosphorylated Protein Fixation/Permeabilization Kit (PF00026). This data was developed using the same antibody clone with 82762-11-PBS in a different storage buffer formulation.

Phospho-HER2/ErbB2 (Tyr877) Uni-rAb^® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA

Cat No. 82762-11-PBS

Clone No.250539F5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

ErbB2 (Tyr877), HER2 (Tyr877), HER2/ErbB2 (Tyr877), P-HER2/ErbB2, P-HER2/ErbB2 (Tyr877)

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Product Information

82762-11-PBS targets Phospho-HER2/ErbB2 (Tyr877) in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian)
• Calculated Molecular Weight: 1255 aa, 138 kDa
• Observed Molecular Weight: 185 kDa
• GenBank Accession Number: BC156755
• Gene Symbol: HER2/ErbB2
• Gene ID (NCBI): 2064
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04626
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HER2, also known as ErbB2 and Neu, is a 185-kDa transmembrane glycoprotein that is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. Phospho-HER2/ErbB2 (Tyr877) refers to the phosphorylated form of the human epidermal growth-factor receptor 2 (HER2, ErbB2) at tyrosine 877 within its kinase domain. This phosphorylation event is catalyzed in trans by dimerization-activated HER2 itself or by Src-family kinases (SFKs) upon receptor clustering, and serves as a critical activation switch for downstream signaling cascades. ( PMID: 34107301)