Phospho-INPPL1 (Tyr986/987) Polyclonal antibody

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  WB analysis using 29574-1-AP

  Non-treated and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

• 

  WB analysis using 29574-1-AP

  Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

Phospho-INPPL1 (Tyr986/987) Polyclonal Antibody for WB, ELISA

Cat No. 29574-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

INPPL 1, INPPL1, Phospho-INPPL1 (Tyr986/987), Protein 51C, SHIP 2, SHIP2

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Tested Applications

• Positive WB detected in: UV treated HeLa cells, UV treated NIH/3T3 cells, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29574-1-AP targets Phospho-INPPL1 (Tyr986/987) in WB, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: inositol polyphosphate phosphatase-like 1
• Calculated Molecular Weight: 1258 aa, 139 kDa
• Observed Molecular Weight: 150 kDa
• GenBank Accession Number: BC140853
• Gene Symbol: INPPL1
• Gene ID (NCBI): 3636
• RRID: AB_3085347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O15357
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone. It has also been shown that SHIP2 can be phosphorylated at Tyr-986 in COS-7 cells and Chinese hamster ovary cells overexpressing the insulin receptor. (PMID: 11349134)