Phospho-IRAK4 (Thr345/Ser346) Recombinant antibody

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  WB analysis of Karpas-299 using 85753-1-RR

  Non-treated Karpas-299 cells and IL-1 beta treated Karpas-299 cells were subjected to SDS PAGE followed by western blot with 85753-1-RR (Phospho-IRAK4 (Thr345/Ser346) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-IRAK4 (Thr345/Ser346) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85753-1-RR

Clone No.242271A8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

IRAK4 (Ser346), IRAK4 (Thr345), IRAK4 (Thr345/Ser346), P IRAK4 (Thr345/Ser346), Phospho IRAK4 (Thr345/Ser346)

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Tested Applications

• Positive WB detected in: IL-1 beta treated Karpas-299 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85753-1-RR targets Phospho-IRAK4 (Thr345/Ser346) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: interleukin-1 receptor-associated kinase 4
• Calculated Molecular Weight: 52 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC013316
• Gene Symbol: IRAK4
• Gene ID (NCBI): 51135
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NWZ3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333)