Tested Applications
| Positive WB detected in | IL-1 beta treated Karpas-299 cells |
| Positive IHC detected in | rat lymph node tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85753-1-RR targets Phospho-IRAK4 (Thr345/Ser346) in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | interleukin-1 receptor-associated kinase 4 |
| Calculated Molecular Weight | 52 kDa |
| Observed Molecular Weight | 55 kDa |
| GenBank Accession Number | BC013316 |
| Gene Symbol | IRAK4 |
| Gene ID (NCBI) | 51135 |
| RRID | AB_3744347 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NWZ3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IRAK4 is the central kinase in the Toll-like receptor pathway of the innate immune system. Toll-like receptors constitute the first line of defense against pathogenic micro-organisms such as bacteria, viruses, and yeast. Unphosphorylated full-length IRAK4 can autophosphorylate in vitro in a cell-free system. In the cellular context, however, autophosphorylation is inducible by IL-1β. Four phosphorylation sites have been identified in the activation loop in some articles, which are the results of autophosphorylation: Thr-342, Thr-345, Ser-346 and Thr-352. (PMID: 24567333)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Phospho-IRAK4 (Thr345/Ser346) antibody 85753-1-RR | Download protocol |
| WB protocol for Phospho-IRAK4 (Thr345/Ser346) antibody 85753-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-IRAK4 (Thr345/Ser346) Recombinant monoclonal antibody (242271A8), catalog number 85753-1-RR, has 1 total citation. The cited publication appeared in Cell Reports (IF 7.7), published in January 2026. The study investigated how the aromatic microbial metabolite hippuric acid enhances inflammatory responses in macrophages via TLR-MyD88 signaling and lipid remodeling. The research field encompasses innate immunity, Toll-like receptor signaling pathways, macrophage inflammation, and lipid metabolism. The antibody was used for Western blot in mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Rep Aromatic microbial metabolite hippuric acid enhances inflammatory responses in macrophages via TLR-MyD88 signaling and lipid remodeling. |
